Biodiesel from neem oil had been a promising alternative for diesel for the future. In few decades the world energy demand had been increased drastically. The depletion of fossil fuels is the main reason for the research of alternative fuels for internal combustion engines. Biofuels are the greener energy resources which can be used instead of fossil fuels. Biofuels are available plenty in the world and also they are renewable source of energy. In the present study, an experimental work had been carried out to analyze the performance and combustion parameters of B20 neem blend by varying injection ressure, injection timing and nozzle hole diameter.