Neil J. Scolding / David Gordon (ed.)

Neural Cell Transplantation

Methods and Protocols
Herausgegeben:Scolding, Neil J.; Gordon, David

• Gebundenes Buch

Produktbeschreibung

In the dramatic and rapidly developing field of neural transplantation for CNS repair, the most powerful contributor has been the vital research focusing on stem cells. In Neural Cell Transplantation: Methods and Protocols, leading experts in the field examine tried-and-true laboratory techniques in order to supply scientists with a firm foundation upon which further advancements can be based. Written in the highly successful Methods in Molecular Biology(TM) series format, the chapters of this volume include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and Notes sections, which examine tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and easy to use, Neural Cell Transplantation: Methods and Protocols provides the most thorough and essential protocols that will allow new generations of neuroscientists to enter and contribute to this uniquely inspiring field.

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Produktdetails

• Methods in Molecular Biology 549
• Verlag: Humana / Humana Press / Springer, Berlin
• Artikelnr. des Verlages: 12237234, 978-1-60327-930-7
• 2009 edition
• Seitenzahl: 226
• Erscheinungstermin: 7. Mai 2009
• Englisch
• Abmessung: 269mm x 195mm x 20mm
• Gewicht: 723g
• ISBN-13: 9781603279307
• ISBN-10: 160327930X
• Artikelnr.: 25921440

Inhaltsangabe

Introductory Reviews.- Neural Transplantation and Stem Cells.- Adult Stem Cells for the Treatment of Neurological Disease.- Human Trials for Neurodegenerative Disease.- Practical/Methodological Chapters.- Differentiation of Neuroepithelia from Human Embryonic Stem Cells.- Derivation of High-Purity Oligodendroglial Progenitors.- Flow Cytometric Characterization of Neural Precursor Cells and Their Progeny.- Isolation, Expansion, and Differentiation of Adult Mammalian Neural Stem and Progenitor Cells Using the Neurosphere Assay.- Human Mesenchymal Stem Cell Culture for Neural Transplantation.- Umbilical Cord Blood Cells.- Animal Models of Neurodegenerative Diseases.- Animal Models of Multiple Sclerosis.- Transplantation of Oligodendrocyte Progenitor Cells in Animal Models of Leukodystrophies.- A Rat Middle Cerebral Artery Occlusion Model and Intravenous Cellular Delivery.- MR Tracking of Stem Cells in Living Recipients.- Identifying Neural Progenitor Cells in the Adult Brain.- Immune Ablation Followed by Autologous Hematopoietic Stem Cell Transplantation for the Treatment of Poor Prognosis Multiple Sclerosis.

Rezensionen

From the reviews: "In this book, the authors discuss the current understanding of the management of degenerative diseases of the CNS. ... Neurologists, neurosurgeons, neurophysiologists, and neuroscientists are the intended audience. ... the book flows easily and describes the most common research and technical protocols associated with human stem cell handling and separation. It is a well-referenced and illustrated book and worth its value for most labs ... . a good book for neuroscientists who are directly involved in the separation and production of human stem cells." (Celso Agner, Doody's Review Service, December, 2009)