Natural plant dyes have gained worldwide interest and give encouraging tissue staining results when tested. This book aimed to assess the quality of staining of different tissues by Hematoxylin and curcuma longa solutions compared to Hematoxylin and Eosin routine stain, tissue biopsies were obtained from a different tissues of a healthy rabbit and stained using Curcuma Longa solution instead of eosin in the different Hematoxylin-eosin protocols. Curcuma longa extract is a promising histological dye that effectively can replace Eosin stain in the Hematoxylin &Eosin staining protocol.