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This textbook provides a detailed view of the Research in Cryopreservation of buffalo oocytes and embryos from traditional cryopreservation to a new methods of cryopreservation by stepwise vitrification method and In Situ oocyte cryopreservation and thier effect on development of oocytes or embryos post-thawing. It is hoped that the contents will be founded useful and useful to those privileged to work in this fascinating branch of veterinary science.