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There are many well controlled studies documenting that Nigella sativa (Black Cumin) , a medicinal plant, has various pharmacological effects such as in dermatology, rheumatology, hematology. The present work focused on the protective effect of Nigella sativa against isoniazid induced hepatotoxicity in the rabbits. Nigella sativa, in the present study, was found effective in protecting the liver from isoniazid toxicity as seen by histopathological examination and by evaluating the changes in liver enzymes. The hepatoprotective effect of Nigella sativa is attributed mainly to its antioxidant potentials.