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Since millennia physical & mental health have been given pivotal importance. In the systems of Allopathic, Ayurvedic, Homeopathic & Unani, main importance was given to physical & mental health. In Ayurvedic system of medicine a drug popularly known as "Sanjivani Booti" was given ample importance because of its therapeutic effects. Punica granatum has lots of therapeutic activities but there is no much work has been done on the formulation aspect of the particular constituent i.e. Punicalagin, therefore, this research work has been done to find out the effect of formulation by in vivo studies. Punicalagin was extracted,isolated and purified from peels of Punica granatum. To find out effect of Punicalagin,it is formulated into vesicular system and optimized. This optimized formulation is formulated into topical gel to deliver the Punicalagin topically for the wound healing. Various tests and evaluation parameters are done. On the basis of the findings, it can safely be stated that when the drug is delivered through vesicular system, it offers better results by improving the penetration of drug into skin, prolonging the release of drug and delivering the drug to the specific site.