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A novel strategy to fabricate L- Tryosine sensors was developed based on poly(3,4-ethylene dioxythiophene) PEDOT/ZrO2/reduced graphene oxide composite film on glassy carbon electrode. The PEDOT nanotubes were synthesized by using the complex of EDOT Monomer, Acetonitrile, Ammonium oxydisulphate template. Followed by thing coating of PEDOT have been performed by chemical polymerization of EDOT monomer over the ZrO2/rGO to form PEDOT / ZrO2 / rGO nanocomposite. The surface area of the PEDOT, and PEDOT/ZrO2, PEDOT/rGO, ZrO2, rGO, PEDOT / ZrO2 / rGO nanocomposite modified electrodes were investigated using cyclic voltammetry (CV). The resulting above composite was characterized by X - Ray Diffraction (XRD), cyclic voltammetry (CV), electrochemical impedance spectroscopy and linear sweep voltammetry (LSV). Choronoamperometry (CA) The nanocomposite with high surface area of PEDOT / ZrO2 / rGO nano composite film has been highly catalytic activity toward the electrochemical oxidation ofL- Tyrosine.