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In anoxic environments, many aromatic compounds (AC) are transformed to benzoylCoA, prior to dearomatization and ring cleavage. Due to the resonance stability of the aromatic ring, AC are difficult to degrade in the absence of O2. Benzoate degradation in methanogenic ecosystems is mediated by syntrophic association of a fermentative bacterium with a H2/formate-using methanogen. The metabolism of crotonate in the strictly anaerobic bacteria S.aciditrophicus (SB) was studied and indicated that cyclohexane carboxylate and acetate as end products. In addition to benzoate fermentation, SB as the…mehr

Produktbeschreibung
In anoxic environments, many aromatic compounds (AC)
are transformed to benzoylCoA, prior to
dearomatization and ring cleavage. Due to the
resonance stability of the aromatic ring, AC are
difficult to degrade in the absence of O2. Benzoate
degradation in methanogenic ecosystems is mediated by
syntrophic association of a fermentative bacterium
with a H2/formate-using methanogen. The metabolism of
crotonate in the strictly anaerobic bacteria
S.aciditrophicus (SB) was studied and
indicated that cyclohexane carboxylate and acetate as
end products. In addition to benzoate fermentation,
SB as the ability to use benzoate as an electron
acceptor with crotonate as the electron
donor.Phototrophic and denitrifying bacteria couple
the hydrolysis of 2 ATP to reduce benzoylCoA to
cyclohexediene carboxylCoA. The use of
such an energy intensive reaction by fermentative
bacteria such as SB has been questioned since it is
not clear how net ATP synthesis would occur. The
utilization of fluorobenzoate by SB allowed the
detection of a fluorinated diene intermediate.
Proteomic analysis of SB grown with crotonate or
benzoate allowed the identification of gene
products involved in benzoate metabolism.
Autorenporträt
Housna Mouttaki, PhD: Studied Environmental Microbiology at the
University of Oklahoma, USA in Prof. McInerney s laboratory; BS:
Cell Biology and Physiology at Université Blaise Pascal in
Clermont-Ferrand, France.