In this work, three nanocomposite materials including, Au-ZnOx nanocomposite materials, Pt50Ni25Co25 and Pt63Ni31Co6 were prepared by hydrothermal and one-pot methods. Based on these materials, three kinds of electrochemical sensors were constructed and used to detect glucose, catechol, and hydroquinone. A new electrochemical method for hydrogen peroxide sensing was developed. The research results of this work can enrich the research content of electrochemical sensing, and can also provide a reference for the detection of glucose in the blood.