Octyl Glucoside
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Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Octyl glucoside (n-octyl- -D-glucoside) is a detergent frequently used to dissolve integral membrane proteins for studies in biochemistry. Like Genapol X-100 and Triton X-100, it is a nonphysiological amphiphile that makes lipid bilayers less "stiff". It has become one of the most important detergents for purification of membrane proteins because it can readily be removed from final protein extracts. Above its critical micelle concentration of 0.7%, it was noted as…mehr

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Please note that the content of this book primarily consists of articles available from Wikipedia or other free sources online. Octyl glucoside (n-octyl- -D-glucoside) is a detergent frequently used to dissolve integral membrane proteins for studies in biochemistry. Like Genapol X-100 and Triton X-100, it is a nonphysiological amphiphile that makes lipid bilayers less "stiff". It has become one of the most important detergents for purification of membrane proteins because it can readily be removed from final protein extracts. Above its critical micelle concentration of 0.7%, it was noted as the best detergent for improving selectivity of immunoprecipitation of phosphotyrosine modified proteins. The compound gained popularity with researchers following the publication of an improved synthesis in 1978. However, in 1990 the cost remained prohibitive for large-scale protein isolation.