Odorant Binding and Chemosensory Proteins

Herausgegeben:Pelosi, Paolo; Knoll, Wolfgang

• Gebundenes Buch

Produktbeschreibung

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field.

Produktdetails

• Methods in Enzymology Volume 642
• Verlag: Academic Press / Elsevier Science & Technology
• Artikelnr. des Verlages: S0076-6879(20)X0014-0
• Englisch
• Abmessung: 27mm x 152mm x 229mm
• Gewicht: 930g
• ISBN-13: 9780128211571
• Artikelnr.: 67579417

Autorenporträt

Paolo Pelosi is at AIT Austrian Institute of Technology GmbH, Austria

Inhaltsangabe

1. Genome mining and sequence analysis of chemosensory soluble proteins in arthropods


Joel Vizueta, Paula Escuer, Alejandro Sánchez-Gracia and Julio Rozas




2. Reconstructing protein coding sequences from ancient DNA


Michael Hofreiter and Stefanie Hartmann




3. Bioinformatic, genomic and evolutionary analysis of genes: A case study in Dipteran CSPs


Jean-François Picimbon




4. Proteomics of arthropod soluble olfactory proteins


Francesca R. Dani and Giuseppe Pieraccini




5. Analysis of post-translational modifications in soluble proteins involved in chemical communication from mammals and insects


Andrea Scaloni




6. Bacterial expression and purification of vertebrate odorant-binding proteins


Marine Brulé, Margot Glaz, Christine Belloir, Nicolas Poirier, Lucie Moitrier, Fabrice Neiers and Loïc Briand




7. Structure of odorant binding proteins and chemosensory proteins determined by X-ray crystallography


Anaïs Gaubert, Béatrice Amigues, Silvia Spinelli and Christian Cambillau




8. Solution structure of insect CSP and OBPs by NMR


Serena Leone, Alessandro Emendato, Roberta Spadaccini and Delia Picone




9. Stability of OBPs


Nadja Hellmann




10. Ligand-binding assays with OBPs and CSPs


Chiara D'Onofrio, Valeriia Zaremska, Jiao Zhu, Wolfgang Knoll and Paolo Pelosi




11. Fine structure immunocytochemistry-An important tool for research on odorant-binding proteins


Rudolf Alexander Steinbrecht




12. Effect of OBPs on the response of olfactory receptors


Bing Wang, Song Cao, Wei Liu and Guirong Wang




13. Site-directed mutagenesis of odorant-binding proteins


Jiao Zhu, Valeriia Zaremska, Chiara D'Onofrio, Wolfgang Knoll and Paolo Pelosi




14. Silencing of OBP genes: Generation of loss-of-function mutants of PBP by genome editing


Yusuke Shiota and Takeshi Sakurai




15. Electrochemical biosensing with odorant binding proteins


Sabine Szunerits, Rabah Boukherroub and Alina Vasilescu




16. Electronic biosensors based on graphene FETs


Patrik Aspermair, Vladyslav Mishyn, Sabine Szunerits and Wolfgang Knoll




17. Electronic biosensors based on EGOFETs


Mohammad Y. Mulla, Luisa Torsi and Kyriaki Manoli




18. Gravimetric biosensors


Khasim Cali, Elena Tuccori and Krishna C. Persaud




19. Plasmonic biosensors relying on biomolecular conformational changes: Case of odorant binding proteins

Anil Bozdogan, Simone Hageneder and Jakub Dostalek