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The experimental work carried out in this study shows that natural synthesis polyurethane and vegetable waste luffa could be used in a commercial scale for oil adsorption from sea water surface. In this procedure many factors were studied which effect on the oil adsorption capacity (gram oil / gram adsorbent) of polyurethane and luff when they were used as adsorbent in oil spill cleanup. These factors are: Type of water, weight of Adsorbent, shaking time, oil volume, temperature, Water hydrophobicity - oil oleophilicity of adsorbent, PH, and Reusability of Adsorbents. Removal efficiency of oil was studied using seawater and tap water. Efficiency of removal of oil and adsorption capacity was higher for sea water than tap water. The adsorbents weight of 0.2 gram polyurethane and 0.5gram luffa are the optimum amount of adsorbed oil and acceptable amount of water pick up with all types of used oil, so they were used in the experiments. The increase in shaking time increases the efficiency of removal oil from seawater .The optimum value was achieved at about (15min) for all types of used oil. The increase in oil volume decreases the efficiency of removal oil and the adsorpti