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OpenEpi is a free software package that enables a variety of statistical and epidemiological operations to be carried out without the need for programming skills. Its user-friendly interface enables data to be entered into a table, and results to be presented in a clear manner. Many universities use this software to teach epidemiology and biostatistics. Functionalities include comparison of proportions, percentages and averages, as well as sample size calculation for different types of epidemiological studies. With its 20 introductory modules, this manual is aimed primarily at students and researchers in the biomedical sciences who wish to carry out research work, but do not have the required knowledge of biostatistics. The aim is to help them overcome this hurdle, while offering a simple interpretation of results, enabling them to benefit from the advantages of easy-to-use, practical software.