Guy Cox

Optical Imaging Techniques in Cell Biology

• Broschiertes Buch

Produktbeschreibung

This reference on optical imaging techniques covers the field from the basics of the optical microscope to highly advanced techniques such as Förster resonant energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM). Using a uniform style and format, it provides an understanding of the underlying principles, so readers can learn the benefits and limitations of each technique described. Topics covered include: optical contrasting techniques, fluorescence and fluorescence microscopes, three-dimensional imaging, the confocal microscope, nonlinear microscopy, advanced fluorescence techniques, and breaking the diffraction limit. Chapters include suggestions for further reading.

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Produktdetails

• Verlag: CRC Press
• 2nd edition
• Seitenzahl: 316
• Erscheinungstermin: 23. Oktober 2017
• Englisch
• Abmessung: 234mm x 156mm x 17mm
• Gewicht: 445g
• ISBN-13: 9781138199408
• ISBN-10: 1138199400
• Artikelnr.: 50153683

Autorenporträt

Guy Cox is a professor within the Electron Microscopy Unit at the University of Sydney, Australia.

Inhaltsangabe

The Light Microscope. Optical Contrasting Techniques. Fluorescence and
Fluorescence Microscopy. Image Capture. The Confocal Microscope. The
Digital Image. Aberrations and Their Consequences. Nonlinear Microscopy.
High-Speed Confocal Microscopy. Deconvolution and Image Processing.
Three-Dimensional Imaging: Stereoscopy and Reconstruction. Green
Fluorescent Protein. Fluorescent Staining. Quantitative Fluorescence.
Advanced Fluorescence Techniques: FLIM, FRET, and FCS. Evanescent Wave
Microscopy. Beyond the Diffraction Limit. Appendix A: Microscope Care and
Maintenance. Appendix B: Keeping Cells Alive under the Microscope. Appendix
C: Antibody Labeling of Plant and Animal Cells: Tips and Sample Schedules.
Appendix D: Image Processing with ImageJ.