Growth and survival of Portunus pelagicus juveniles in captivity were investigated with different bottom substrates, feeds and stocking density. The highest survival of the juveniles at 65.33% was achieved (P 0.05) using sandy substrates followed by soil (29.33%), sand + soil (28.00%) and control (21.33%). Water quality parameters were overall better in RAS with a significantly (P 0.05) lower level of ammonia-N (0.04 ± 0.10 mg/L) and nitrite-N (0.02 ± 0.07 mg/L). The crabs fed with squid showed significantly (P 0.05) higher carapace width (68.02 ± 3.51 mm) than those fed the scad fish (53.69 ± 3.32 mm) and Acetes shrimps (51.19 ± 3.76 mm). Feeding three times per day exhibited the highest carapace width (65.23 ± 1.61 mm) and survival (50.03 ± 0.02%) of P. pelagicus juveniles. The highest percentage of survival (58.33 ± 0.02%) was observed at 16 individuals / m2 whereas, the lowest was found at 28 individuals / m2 (33.36 ± 2.62%) in different stocking densities experiment. Therefore, the practice of mixed feeds, feeding frequency of three times per day and 16 individuals / m2 stocking density is recommended for juvenile culture of blue swimming crab