This paper presents natural frequency and stress of opto-mechanical structure. In this study, an opto-mechanical structure was investigated.The opto-mechanical structure includes lens assembly and detector head assembly (DHA). The opto-mechanical structure requires high stability and position accuracy. In this design and analysis study, wide ranges of the opto-mechanical structural analysis have been performed including: Natural frequency analysis and dynamic analysis. Opto-mechanical design includes material selection and mechanical design. Payload structure design will be based on stiffness. Hence, Natural frequency is major design criteria for opto-mechanical design of payload structure. At higher vibration during launching time, resonance will be created and parts will be misaligned or may be damaged. Objective of this work is to reach maximum natural frequency with minimum stress on opto-mechanical structure.