This detailed volume addresses the challenge of how to instruct stem/early progenitor cells to progress through appropriate steps to generate functional 3-dimensional organs, one of the outstanding issues in regenerative medicine. The field of organoids is geared towards defining and demonstrating the in vitro conditions that achieve this goal. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on…mehr
This detailed volume addresses the challenge of how to instruct stem/early progenitor cells to progress through appropriate steps to generate functional 3-dimensional organs, one of the outstanding issues in regenerative medicine. The field of organoids is geared towards defining and demonstrating the in vitro conditions that achieve this goal. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Organoids: Stem Cells, Structure, and Function serves as an aid to researchers working in this vital area of research.
A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment.- Clinically-Amendable Defined and Rapid Induction of Human Brain Organoids from Induced Pluripotent Stem Cells.- Organoid Assay of Human Cancer Stem Cells.- Construction of Thymus Organoids from Decellularized Thymus Scaffolds.- Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres.- Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells.- Organoid Culture of Lingual Epithelial Cells in a Three-Dimensional Matrix.- Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension.- Efficient Culture of Intestinal Organoids with Blebbistatin.- Isolation and Culture of Adult Intestinal, Gastric, and Liver Organoids for Cre-Recombinase-Mediated Gene Deletion.- The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine.- New Trends and Perspectives in theFunction of Non-Neuronal Acetylcholine in Crypt-Villus Organoids in Mice.- Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System.- Murine Colonic Organoid Culture System and Downstream Assay Applications.- Intestinal Organoids as a Novel Tool to Study Microbes-Epithelium Interactions.- The Isolation, Culture, and Propagation of Murine Intestinal Enteroids for the Study of Dietary Lipid Metabolism.- Oncogenic Transformation of Human-Derived Gastric Organoids.- Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation.- Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections.- Study Bacterial-Host Interactions Using Intestinal Organoids.- Disaggregation and Reaggregation of Zebrafish Retinal Cells for the Analysis of Neuronal Layering.- Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo.- Scaffold-Based and Scaffold-Free Testicular Organoids from Primary Human Testicular Cells.- Use of a Super-Hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-Organoids.- Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly.- Cell Microencapsulation in Polyethylene Glycol Hydrogel Microspheres Using Electrohydrodynamic Spraying.- Gastrointestinal Epithelial Organoid Cultures from Post-Surgical Tissues.- Drug Sensitivity Assays of Human Cancer Organoid Cultures.
A Simple Method of Generating 3D Brain Organoids Using Standard Laboratory Equipment.- Clinically-Amendable Defined and Rapid Induction of Human Brain Organoids from Induced Pluripotent Stem Cells.- Organoid Assay of Human Cancer Stem Cells.- Construction of Thymus Organoids from Decellularized Thymus Scaffolds.- Expansion of Human Airway Basal Stem Cells and Their Differentiation as 3D Tracheospheres.- Human Pluripotent Stem Cells (iPSC) Generation, Culture, and Differentiation to Lung Progenitor Cells.- Organoid Culture of Lingual Epithelial Cells in a Three-Dimensional Matrix.- Generation of Functional Kidney Organoids In Vivo Starting from a Single-Cell Suspension.- Efficient Culture of Intestinal Organoids with Blebbistatin.- Isolation and Culture of Adult Intestinal, Gastric, and Liver Organoids for Cre-Recombinase-Mediated Gene Deletion.- The Three-Dimensional Culture of Epithelial Organoids Derived from Embryonic Chicken Intestine.- New Trends and Perspectives in theFunction of Non-Neuronal Acetylcholine in Crypt-Villus Organoids in Mice.- Derivation of Intestinal Organoids from Human Induced Pluripotent Stem Cells for Use as an Infection System.- Murine Colonic Organoid Culture System and Downstream Assay Applications.- Intestinal Organoids as a Novel Tool to Study Microbes-Epithelium Interactions.- The Isolation, Culture, and Propagation of Murine Intestinal Enteroids for the Study of Dietary Lipid Metabolism.- Oncogenic Transformation of Human-Derived Gastric Organoids.- Intestinal Crypt Organoid: Isolation of Intestinal Stem Cells, In Vitro Culture, and Optical Observation.- Human Intestinal Enteroids: New Models to Study Gastrointestinal Virus Infections.- Study Bacterial-Host Interactions Using Intestinal Organoids.- Disaggregation and Reaggregation of Zebrafish Retinal Cells for the Analysis of Neuronal Layering.- Antibody Uptake Assay in the Embryonic Zebrafish Forebrain to Study Notch Signaling Dynamics in Neural Progenitor Cells In Vivo.- Scaffold-Based and Scaffold-Free Testicular Organoids from Primary Human Testicular Cells.- Use of a Super-Hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-Organoids.- Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly.- Cell Microencapsulation in Polyethylene Glycol Hydrogel Microspheres Using Electrohydrodynamic Spraying.- Gastrointestinal Epithelial Organoid Cultures from Post-Surgical Tissues.- Drug Sensitivity Assays of Human Cancer Organoid Cultures.
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