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Fascioliasis and other plant-borne trematodes zoonoses are well known helminths parasites of herbivorous animals which is caused by two trematodes F. hepatica and F. gigantica. This disease is found in many areas. Fascioliasis reduces the production of meat, milk and wool, in addition to losses due to mortalities, liver condemnation and expenditures for antihelminthic using. In Egypt fascioliasis, existed since Pharaonic ages can infect also donkeys and camels as well as herbivorous animals, which will finally infect human leading to serious hepatic pathological sequences. Fascioliasis can be differentially diagnosed from some other diseases as acute hepatitis, schistosomiasis, visceral toxocariasis, biliary tract diseases. This work aims to evaluate an early immunodiagnosis protocol for fascioliasis using sandwich ELISA to Antigen. On the other hand, study the humoral and cellular immune response.