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The ability of bacterial colonies to induce hemolysis when grown on blood agar is used to classify certain microorganisms. They are three types of hemolysis alpha, beta and gamma. Alpha hemolysis is partial hemolysis andcalled green hemolysis because of the color change in the agar.Beta hemolysis is complete hemolysis, is a complete lysis of red cells in the media and appearlightened and transparent.Gamma hemolysis is non-hemolytic. A substance that causes hemolysis is a hemolysin Hemolysins are certain proteins and lipids that cause lysis of red blood cells by damaging their cell membrane.Although hemolysins are able to lyse red blood cells in vitro, the ability of hemolysins to target other cells, including white blood cells, often accounts for the effects of hemolysins during infection. Pathogenic Hemolytic Bacteria may be gram positive bacteria such as (staphylococcus, streptococcus, bacillus and listeria ) Gram negative bacteria such as (Neisseria, Pseudomonas,Haemophilus,Helicobacter, Escherichi, Klebsiella, Salmonella, Shigella and Proteus),Study general characterstics of each bacterium that cause human diseases.