Table of contents:
PCR as a Daily Used Technique in Molecular Biology (J.-P. Charlieu). The Design of Primers for PCR (A. Sharrocks). Purification of PCR Products (L. Mezei and D. Storts). Cloning PCR Products (L. Mezei and D. Storts). Amplifying of Unknown Flanking DNA by Single-Specific-Primer PCR (J. Stappert). Generation of Labeled DNA Probes by PCR (G. Konat). Nonisotopic Probe Generation by PCR (E.P.H. Yap, et al.). Direct PCR Screening of Lambda and Cosmid Libraries (H.G. Griffin). Methods for Generating Multiple Site Directed Mutations In Vitro (J. Stappert). Mutagenesis Using PCR (B. Tao). Direct Automated DNA Sequencing of ds and ss PCR Products (A. Lew). Distinction between Almost Identical DNA Sequences by Polymerase Chain Reaction (J.-P. Charlieu). Detection of Mutations by PCR (E.P.H. Yap, et al.). Mapped Restriction Site Polymorphisms (MRSPs) in PCR Products for Rapid Identification and Classification of Genetically Distinct Organisms (D. Ralph and M. McClelland). Detection of Polym orphic DNA Sequences at the 3' end of Alu Repeats by PCR (J.-P. Charlieu). Ligation-Anchored PCR (A. Troutt). PCR-Limiting Dilution Analysis (A. Troutt). Direct PCR from Whole Blood Using Formamide and Low Temperatures (A. Lew). Immuno-PCR: A Generic Method for Purifying Target for PCR (A. Lew). Nonisotopic Single Stranded Conformation Polymorphism (SSCP) Analysis of PCR Products (E.P.H. Yap, et al.). The Use of PCR-RAPD Analysis in Plant Taxonomy and Evolution (T. Demeke and R.P. Adams). Optimization of DNA Extraction and PCR Procedures for Random Amplified Polymorphic DNA (RAPD) Analysis in Plants (K. Yu and K.P. Pauls). The Use of RAPD Analysis to Tag Genes and Determine Relatedness in Heterologous Plant Populations Using Tetraploid Alfalfa as an Example (K. Yu and K.P. Pauls). DNA Recombination in the Course of PCR (A. Vartapetian). Thermostable DNA Polymerases for In Vitro DNA Amplifications (A. Bej and M. Mahbubani). PCR in Sequence-Tagged Site (STS) Content Genome Mapping (A. Srivastava). False Positives and Contamination in PCR (E.P.H. Yap, et al.). The Application of Polymerase Chain Reaction (PCR) Based Technologies to Forensic Analysis (L. Presley). Application of PCR Amplified DNA Markers in Identification of Individuals (L. Peltonen and A. Sajantila). PCR in Forensic Science (V. Pascali). Applications of Polymerase Chain Reaction Methodology in Clinical Diagnostics (A. Bej and M. Mahbubani). Applications of the Polymerase Chain Reaction (PCR) In Vitro DNA Amplification Method in Environmental Microbiology (A. Bej and M. Mahbubani). Detection of Foodborne Microbial Pathogens Using Polymerase Chain Reaction (D. Jones and A. Bej).
Since Polymerase Chain Reaction (PCR) was first described in 1985, the range of applications of the technique have expanded enormously. This comprehensive, up-to-date volume covers a diverse range of PCR applications, including detection of genetic mutations, detection of foodborne pathogens, forensic applications, determination of relationships among different plant species, generation of labeled probes, screening of lambda and cosmid libraries, and many more. Several chapters deal with the mechanics of the PCR technique itself and give practical advice on such topics as primer design, choice of polymerase, and optimum reaction parameters. The comprehensive nature and practicality of the book make it an important acquisition for both new and experienced researchers in the field.
PCR as a Daily Used Technique in Molecular Biology (J.-P. Charlieu). The Design of Primers for PCR (A. Sharrocks). Purification of PCR Products (L. Mezei and D. Storts). Cloning PCR Products (L. Mezei and D. Storts). Amplifying of Unknown Flanking DNA by Single-Specific-Primer PCR (J. Stappert). Generation of Labeled DNA Probes by PCR (G. Konat). Nonisotopic Probe Generation by PCR (E.P.H. Yap, et al.). Direct PCR Screening of Lambda and Cosmid Libraries (H.G. Griffin). Methods for Generating Multiple Site Directed Mutations In Vitro (J. Stappert). Mutagenesis Using PCR (B. Tao). Direct Automated DNA Sequencing of ds and ss PCR Products (A. Lew). Distinction between Almost Identical DNA Sequences by Polymerase Chain Reaction (J.-P. Charlieu). Detection of Mutations by PCR (E.P.H. Yap, et al.). Mapped Restriction Site Polymorphisms (MRSPs) in PCR Products for Rapid Identification and Classification of Genetically Distinct Organisms (D. Ralph and M. McClelland). Detection of Polym orphic DNA Sequences at the 3' end of Alu Repeats by PCR (J.-P. Charlieu). Ligation-Anchored PCR (A. Troutt). PCR-Limiting Dilution Analysis (A. Troutt). Direct PCR from Whole Blood Using Formamide and Low Temperatures (A. Lew). Immuno-PCR: A Generic Method for Purifying Target for PCR (A. Lew). Nonisotopic Single Stranded Conformation Polymorphism (SSCP) Analysis of PCR Products (E.P.H. Yap, et al.). The Use of PCR-RAPD Analysis in Plant Taxonomy and Evolution (T. Demeke and R.P. Adams). Optimization of DNA Extraction and PCR Procedures for Random Amplified Polymorphic DNA (RAPD) Analysis in Plants (K. Yu and K.P. Pauls). The Use of RAPD Analysis to Tag Genes and Determine Relatedness in Heterologous Plant Populations Using Tetraploid Alfalfa as an Example (K. Yu and K.P. Pauls). DNA Recombination in the Course of PCR (A. Vartapetian). Thermostable DNA Polymerases for In Vitro DNA Amplifications (A. Bej and M. Mahbubani). PCR in Sequence-Tagged Site (STS) Content Genome Mapping (A. Srivastava). False Positives and Contamination in PCR (E.P.H. Yap, et al.). The Application of Polymerase Chain Reaction (PCR) Based Technologies to Forensic Analysis (L. Presley). Application of PCR Amplified DNA Markers in Identification of Individuals (L. Peltonen and A. Sajantila). PCR in Forensic Science (V. Pascali). Applications of Polymerase Chain Reaction Methodology in Clinical Diagnostics (A. Bej and M. Mahbubani). Applications of the Polymerase Chain Reaction (PCR) In Vitro DNA Amplification Method in Environmental Microbiology (A. Bej and M. Mahbubani). Detection of Foodborne Microbial Pathogens Using Polymerase Chain Reaction (D. Jones and A. Bej).
Since Polymerase Chain Reaction (PCR) was first described in 1985, the range of applications of the technique have expanded enormously. This comprehensive, up-to-date volume covers a diverse range of PCR applications, including detection of genetic mutations, detection of foodborne pathogens, forensic applications, determination of relationships among different plant species, generation of labeled probes, screening of lambda and cosmid libraries, and many more. Several chapters deal with the mechanics of the PCR technique itself and give practical advice on such topics as primer design, choice of polymerase, and optimum reaction parameters. The comprehensive nature and practicality of the book make it an important acquisition for both new and experienced researchers in the field.