Henry Erlich

PCR Technology

Principles and Applications for DNA Amplification

• Broschiertes Buch

Produktbeschreibung

This is an introduction to the methods and applications of polymerase chain reaction (PCR) technology, a technology developed by Erlich's group at Cetus and Cetus, and is expected to be used in all biology laboratories worldwide within the next few years.

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Produktdetails

• Verlag: Palgrave Macmillan / Palgrave Macmillan UK / Springer Palgrave Macmillan
• Artikelnr. des Verlages: 978-0-333-48948-2
• 1989
• Seitenzahl: 246
• Erscheinungstermin: 1. Oktober 1989
• Englisch
• Abmessung: 228mm x 150mm x 16mm
• Gewicht: 387g
• ISBN-13: 9780333489482
• ISBN-10: 0333489489
• Artikelnr.: 25024216

Inhaltsangabe

Part 1 Introduction to the PCR: history of PCR development; description of the PCR method - basic principles and properties of the PCR; thermostable DNA polymerases; automation; sample preparation. Part 2 Research applications - brief overview: direct sequencing using PCR; preparation of specific DNA fragments using PCR; detection of gene expression; genetic mapping using PCR amplification of individual gametes. Part 3 Diagnostic applications - brief overview: detection of pathogens; diagnosis of monogenic genetic diseases; HLA DNA typing and disease susceptibility; forensic applications; detection of activated oncogenes; future developments and applications.