Peptidomimetic building blocks - Breitung, Sven
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A well-known example of regulatory RNA-protein interaction is the complex of TAR-RNA of HIV-1 and the viral protein Tat. This complex is important for the efficient transcription of the viral genome. Knowledge of the principles of Tat to TAR RNA recognition should make it possible to design specific ligands that can attack as antiviral Tat antagonists. The aim of this thesis was the synthesis of RNA ligands for solid phase peptide synthesis (FPPS) based on heteroaromatic building blocks. The binding affinities of peptides and small molecules from the FPPS to the TAR RNA of HIV-1 were…mehr

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Produktbeschreibung
A well-known example of regulatory RNA-protein interaction is the complex of TAR-RNA of HIV-1 and the viral protein Tat. This complex is important for the efficient transcription of the viral genome. Knowledge of the principles of Tat to TAR RNA recognition should make it possible to design specific ligands that can attack as antiviral Tat antagonists. The aim of this thesis was the synthesis of RNA ligands for solid phase peptide synthesis (FPPS) based on heteroaromatic building blocks. The binding affinities of peptides and small molecules from the FPPS to the TAR RNA of HIV-1 were determined by fluorescence assay and fluorescence correlation spectroscopy (FCS). NMR studies and molecular dynamic calculations made it possible to detect a conformational change in TAR RNA. Antiviral properties of peptides and small molecules were determined by a HeLa P4 cell assay. A further goal of the work was the investigation of the binding concept and the antiviral properties of diaminopyrazoles and indazoles.
Autorenporträt
Sven Breitung se doctoró en la Universidad Goethe de Frankfurt am Main en síntesis y análisis de ligandos de ARN. Tras su paso por el Instituto de Química Orgánica y Biología Química, se incorporó a la Organic Electronics Association (OE-A), un grupo de trabajo de la VDMA, como director de proyectos.