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The personality and gender of every individual constitute the very key aspects of their being in existence. This profound thought inspired the study to ferret into the influence of personality and gender on career choice of tertiary students. Analysis of data revealed that, in the Ghanaian context; personality and gender does not have any significant effect on career choice. However, other factors like culture, parental influence, family business, course grades, peer influence and economic hardships rather were the standing reasons why we had this insignificance. The contradiction in findings of this study compared to those of the western literature exposes the inconsistencies in this area and may require that, future researchers adopt a qualitative approach to discover the possible factors that had led to those findings in the Ghanaian setting. Also Parents, teachers, mentors are therefore urged to develop appropriate means that can help them appreciate the student's interest, skills and personality so as to support them in harnessing them better.