This manual is an effort for students and scholars related to Pharmacy or Life science to understand the basic principles and procedures of Microbiology. Pharmaceutical Microbiology is a part of Microbiology with the core application to obtain pharmaceuticals which should be safe to use. The text is designed in a simple way to understand the concepts of Microbiology in order to be clear with the concepts thereby helping them to apply them in the field of research. The book contains relevant data with respect to screening, isolation, identification, cultivation and preservation of microbes; microbiological assay of antibiotics and vitamins; and sterility testing of pharmaceuticals.