Adenosine 3 ,5 -cyclic monophosphate (cAMP) and guanosine 3 ,5 -cyclic monophosphate are ubiquitous nucleotides that have been described as the first and second messengers. In concert with intracellular calcium and IP3, they play a major role in the control of intracellular signaling, which orchestrates normal and pathophysiological responses. Downstream from the cyclic nucleotide synthesis by adenylyl and guanylyl cyclases, the multigenic family of cyclic nucleotide phosphodiesterases (PDEs), by specifically hydrolyzing cyclic nucleotides, controls cAMP and cGMP levels to maintain a basal…mehr
Adenosine 3 ,5 -cyclic monophosphate (cAMP) and guanosine 3 ,5 -cyclic monophosphate are ubiquitous nucleotides that have been described as the first and second messengers. In concert with intracellular calcium and IP3, they play a major role in the control of intracellular signaling, which orchestrates normal and pathophysiological responses. Downstream from the cyclic nucleotide synthesis by adenylyl and guanylyl cyclases, the multigenic family of cyclic nucleotide phosphodiesterases (PDEs), by specifically hydrolyzing cyclic nucleotides, controls cAMP and cGMP levels to maintain a basal state. Their critical role in intracellular sign- ing has recently designated them as new therapeutical targets. Several leading pharmaceutical companies are searching and developing new therapeutic agents that would potently and selectively inhibit PDE isozymes, notably PDE4 and PDE5. Nevertheless, the precise mechanism and the contribution of the various PDE isozymes in modulating intracellular signaling remain to be established. The aim of Phosphodiesterase Methods and Protocols is to provide a palette of a variety of conceptual and technical approaches designed to solve qu- tions concerning the role of PDEs, and ultimately of their different variants, in physiological functions as well as their implications in several pathologies.
Study of Cyclic Adenosine Monophosphate Microdomains in Cells Marco Mongillo, Anna Terrin, Sandrine Evellin, Valentina Lissandron, and Manuela Zaccolo High-Resolution Measurements of Cyclic Adenosine Monophosphate Signals in 3D Microdomains Jeffrey W. Karpen and Thomas C. Rich Cygnets: In Vivo Characterization of Novel cGMP Indicators and In Vivo Imaging of Intracellular cGMP Akira Honda, Carolyn L. Sawyer, Sharon M. Cawley, and Wolfgang R. G. Dostmann High-Throughput Screening of Phosphodiesterase Activity in Living Cells Thomas C. Rich and Jeffrey W. Karpen Assessment of Phosphodiesterase Isozyme Contribution in Cell and Tissue Extracts Thérèse Keravis, Rima Thaseldar-Roumié, and Claire Lugnier Localization of the Cyclic Guanosine 39,59-Monophosphate- Hydrolyzing Phosphodiesterase Type 9 in Rat Brain by Nonradioactive In Situ Hybridization Wilma C. G. van Staveren and Marjanne Markerink-van Ittersum Determination of Ca2+/Calmodulin-Stimulated Phosphodiesterase Activity in Intact Cells Chen Yan Adenovirus-Mediated Overexpression of Murine Cyclic Nucleotide Phosphodiesterase 3B Faiyaz Ahmad, Linda Härndahl, Yan Tang, Lena Stenson Holst, and Vincent C. Manganiello Identification of Promoter Elements in the 5'-Flanking Region of Murine Cyclic Nucleotide Phosphodiesterase 3B Gene Hanguan Liu, Jing Rong Tang, Eva Degerman, and Vincent C. Manganiello Purification of PDE6 Isozymes From Mammalian Retina Dana C. Pentia, Suzanne Hosier, Rachel A. Collupy, Beverly A. Valeriani, and Rick H. Cote Cyclic Guanosine 59-Monophosphate Binding to Regulatory GAF Domains of Photoreceptor Phosphodiesterase Rick H. Cote Renaturation of the Catalytic Domain of PDE4A Expressed in Escherichia coli as Inclusion Bodies Wito Richter, Thomas Hermsdorf, and Dietrich Dettmer Determining the Subunit Structure of Phosphodiesterases Using Gel Filtration and Sucrose Density Gradient Centrifugation Wito Richter Crystallization of Cyclic Nucleotide Phosphodiesterases Hengming Ke, Qing Huai, and Robert X. Xu Generation of PDE4 Knockout Mice by Gene Targeting S.-L. Catherine Jin, Anne M. Latour, and Marco Conti Immunoprecipitation of PDE2 Phosphorylated and Inactivated by an Associated Protein Kinase J. Kelley Bentley Investigation of Extracellular Signal-Regulated Kinase 2 Mitogen-Activated Protein Kinase Phosphorylation and Regulation of Activity of PDE4 Cyclic Adenosine Monophosphate-Specific Phosphodiesterases Elaine V. Hill, Miles D. Houslay, and George S. Baillie Radiolabeled Ligand Binding to the Catalytic or Allosteric Sites of PDE5 and PDE11 James L. Weeks II, Mitsi A. Blount, Alfreda Beasley, Roya Zoraghi, Melissa K. Thomas, Konjeti Raja Sekhar, Jackie D. Corbin, and Sharron H. Francis Analysis of Dimerization Determinants of the PDE6 Catalytic Subunits Khakim G. Muradov, Kimberly K. Boyd, and Nikolai O. Artemyev Interaction Between Catalytic and Inhibitory Subunits of PDE6 Nikolai O. Artemyev Purification, Reconstitution on Lipid Vesicles, and Assays of PDE6and Its Activator G Protein, Transducin Theodore G. Wensel, Feng He, and Justine A. Malinski Index
Study of Cyclic Adenosine Monophosphate Microdomains in Cells Marco Mongillo, Anna Terrin, Sandrine Evellin, Valentina Lissandron, and Manuela Zaccolo High-Resolution Measurements of Cyclic Adenosine Monophosphate Signals in 3D Microdomains Jeffrey W. Karpen and Thomas C. Rich Cygnets: In Vivo Characterization of Novel cGMP Indicators and In Vivo Imaging of Intracellular cGMP Akira Honda, Carolyn L. Sawyer, Sharon M. Cawley, and Wolfgang R. G. Dostmann High-Throughput Screening of Phosphodiesterase Activity in Living Cells Thomas C. Rich and Jeffrey W. Karpen Assessment of Phosphodiesterase Isozyme Contribution in Cell and Tissue Extracts Thérèse Keravis, Rima Thaseldar-Roumié, and Claire Lugnier Localization of the Cyclic Guanosine 39,59-Monophosphate- Hydrolyzing Phosphodiesterase Type 9 in Rat Brain by Nonradioactive In Situ Hybridization Wilma C. G. van Staveren and Marjanne Markerink-van Ittersum Determination of Ca2+/Calmodulin-Stimulated Phosphodiesterase Activity in Intact Cells Chen Yan Adenovirus-Mediated Overexpression of Murine Cyclic Nucleotide Phosphodiesterase 3B Faiyaz Ahmad, Linda Härndahl, Yan Tang, Lena Stenson Holst, and Vincent C. Manganiello Identification of Promoter Elements in the 5'-Flanking Region of Murine Cyclic Nucleotide Phosphodiesterase 3B Gene Hanguan Liu, Jing Rong Tang, Eva Degerman, and Vincent C. Manganiello Purification of PDE6 Isozymes From Mammalian Retina Dana C. Pentia, Suzanne Hosier, Rachel A. Collupy, Beverly A. Valeriani, and Rick H. Cote Cyclic Guanosine 59-Monophosphate Binding to Regulatory GAF Domains of Photoreceptor Phosphodiesterase Rick H. Cote Renaturation of the Catalytic Domain of PDE4A Expressed in Escherichia coli as Inclusion Bodies Wito Richter, Thomas Hermsdorf, and Dietrich Dettmer Determining the Subunit Structure of Phosphodiesterases Using Gel Filtration and Sucrose Density Gradient Centrifugation Wito Richter Crystallization of Cyclic Nucleotide Phosphodiesterases Hengming Ke, Qing Huai, and Robert X. Xu Generation of PDE4 Knockout Mice by Gene Targeting S.-L. Catherine Jin, Anne M. Latour, and Marco Conti Immunoprecipitation of PDE2 Phosphorylated and Inactivated by an Associated Protein Kinase J. Kelley Bentley Investigation of Extracellular Signal-Regulated Kinase 2 Mitogen-Activated Protein Kinase Phosphorylation and Regulation of Activity of PDE4 Cyclic Adenosine Monophosphate-Specific Phosphodiesterases Elaine V. Hill, Miles D. Houslay, and George S. Baillie Radiolabeled Ligand Binding to the Catalytic or Allosteric Sites of PDE5 and PDE11 James L. Weeks II, Mitsi A. Blount, Alfreda Beasley, Roya Zoraghi, Melissa K. Thomas, Konjeti Raja Sekhar, Jackie D. Corbin, and Sharron H. Francis Analysis of Dimerization Determinants of the PDE6 Catalytic Subunits Khakim G. Muradov, Kimberly K. Boyd, and Nikolai O. Artemyev Interaction Between Catalytic and Inhibitory Subunits of PDE6 Nikolai O. Artemyev Purification, Reconstitution on Lipid Vesicles, and Assays of PDE6and Its Activator G Protein, Transducin Theodore G. Wensel, Feng He, and Justine A. Malinski Index
Rezensionen
"...interesting and thought provoking, but technically challenging...excellent guide to current research techniques in the field of phosphodiesterase." - Doody's Health Sciences Book Review Journal
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