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The research work was carried out to determine the physico-chemical characteristics and antioxidant assay of 13 selected tomato germplasm collected from the south-western region of Bangladesh during December2012 to May 2013. A significant variation among the germplasm in relation to fruit characteristics was observed. The author is obliged to express his sincere gratitude to supreme personality of Godhead for ending blessing to complete the research work and this book with a sense of devotion and diligence. The author express his deep seance of gratitude, indebtedness and profound respect to his colleague of Agrotechnology Discipline of Khulna University in Bangladesh for their scholastic supervision, helpful advice and constructive criticism throughout the entire period of research work. The author feels a great delight to express his sincere gratitude and profound respect to his beloved parents and friends for their blessing and continuous support.Generous thanks to the personnel of central library, seminar library and laboratory technician from where the author gathered valuable information regarding the study.