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The physicochemical properties and fatty acid composition of Madhuca longifolia (J.Konig) J.F.Macbr. (Mahua) and Semecarpus anacardium Linn. (Bhilamo) seeds oil were investigated. The oil was extracted in a soxhlet apparatus using hexane and evaluated for acid value, iodine value, peroxide value saponification values, reflective index and moisture content. The oil was also analyzed for different element viz. Ag, Al, B, Ba, Bi, Ca, Co, Cr, Cu, K, Li, Mn, Na, Ni, Au,etc using ICPMS. The fatty acid profile of the oil was determined by GC-MS. For each analysis Mahua oil was compared to Bhilamo oil. The physicochemical analysis of Bhilamo oil showed less values viz. acid value as compared to Mahua oil. The different element analysis showed that gold and silver like important elements were found in both the oil at different levels. The qualitative analysis showed very similar trend in their fatty acid composition, in which 15 compounds were found in Mahua oil, while 18 compounds were found in Bhilamo oil. The study suggest that physicochemical properties and fatty acid profile of Bhilamo and Mahua has been done which can be use for further research on these plants.