Present clinical study entitled "A critical study on Muladhara Cakra in Females, its anatomical correlation & clinical significance" comprises of conceptual study of Muladhara Cakra and to expose all the controversies regarding the anatomical structure (organs) related to Muladhara Cakra and its functions. On the basis of which a clinical study was designed to evaluate the role of interventional technique i.e.Mulabandha and Dhyana, affecting the Muladhara Cakra in cases of Uterine prolapse (mild grade) and urinary incontinence.Information generation was exhaustive from all available texts of Ayurveda contemporary texts and existing tools) and texts of Yoga .The tool developed by using modern methods of validation and standardization in reference to Muladhara Cakra and its applied aspect is the first attempt that has offered authentic tool for use by the medical professionals in general and the Àyurveda community in particular. MulaBandha intervention adds new dimensions to modernsystem of medicine to recommend specific Life-style changes with / without medications for prevention and cure of diseases.