A plant type with a good morphoframe would sustain more boll load with synchronous boll development and boll burst.In Bt cotton,the plant morphoframe doesn t develop fully due to retention of early stage squares.In an attempt to improve Bt plant morphoframe through removing early squares,so a field experiment was carried out at the Main Cotton Research Station,NAU, Surat during the Kharif season of year 2008-09,to study Physiological Manipulation of Bt Cotton Morphoframe by using Ethylene. 20 treatment combinations were taken, consisting of 5 hybrids of Cotton (viz. RCH 2 Bt,JKCH 99 Bt,NCEH 2R Bt,Mallika Bt and non-Bt G.Cot.Hy.10) and 2 concentrations of Ethylene(i.e. 30 and 45 ppm) and one time hand removal of squares besides untreated control.Square removal treatments were applied at square initiation(at 45-50 DAS) with the objective of study the temporal and spatial distribution of fruiting forms in Bt and conventional cotton hybrid and to understand the consequence of alteredsink activity on physiology,growth,yield and quality of cotton plant.The response of Cotton to square removal was assessed by measuring various growth,yield and quality contributing characters.