Phytochemical and Antimicrobial Screening
Of the Stem Bark Extract(s) of Indigofera Arrecta Hochst Ex A. Rich (Fabaceae)
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The Pulverized stem bark of Indigofera arrectawas exhaustively extracted with methanol and concentrated in vacuo using rotary evaporator at 40 0C.The extract was later subjected to solvent partitioning to yield soluble extracts of n-hexane, ethyl acetate, chloroform, and methanol. Genernal phytochemical screening of the fractions revealed the presence of secondary metabolites such as cardiac glycoside, steroid, terpenes flavonoids and tannins. The antimicrobial activity against S. aureus, S. pyogenes, S.feacalis, S.typhii, E.coli C. ulcerans, P. vulgaris and C.albicans was tested using the tub...
The Pulverized stem bark of Indigofera arrectawas exhaustively extracted with methanol and concentrated in vacuo using rotary evaporator at 40 0C.The extract was later subjected to solvent partitioning to yield soluble extracts of n-hexane, ethyl acetate, chloroform, and methanol. Genernal phytochemical screening of the fractions revealed the presence of secondary metabolites such as cardiac glycoside, steroid, terpenes flavonoids and tannins. The antimicrobial activity against S. aureus, S. pyogenes, S.feacalis, S.typhii, E.coli C. ulcerans, P. vulgaris and C.albicans was tested using the tube dilution and agar diffusion methods as outlined by the NCCLS. The results of the antimicrobial activity as indicated by the zonesof inhibition of growth of microorganism ranged from 20mm to 40mm for the n-hexane extract, 16mm to 21mm for ethyl acetate extract and 20mm to 27mm for the methanol extract. The MIC result for the n-hexane, ethyl acetate and methanol extracts ranged from 7.5mg/mlto 15mg/ml. The MIC of 15mg/ml exhibited by the n-hexane extract against both gram positive and gram negative bacteria indicates broad spectrum activity of Indigofera arrect.
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