Aims of the work: 1-Determination of calli induction of different explants of different species of Fagonia. 2-Determination of calli induction and calli growth of different explants of Fagonia arabica. 3-Determination of chemical composition of both callus of Fagonia arabica leaf explants and the intact leaf. 4-Determination of antibacterial activity of both callus of Fagonia arabica leaf explants and the intact leaf.