PIPES
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High Quality Content by WIKIPEDIA articles! PIPES is the common name for piperazine-N,N -bis(2-ethanesulfonic acid), a frequently used buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has pKa near the physiological pH which makes it useful in cell culture work. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues. Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer. It has a negligible capacity to bind divalent ions.…mehr

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High Quality Content by WIKIPEDIA articles! PIPES is the common name for piperazine-N,N -bis(2-ethanesulfonic acid), a frequently used buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al. in the 1960s. PIPES has pKa near the physiological pH which makes it useful in cell culture work. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues. Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer. It has a negligible capacity to bind divalent ions.