Michael R. Davey, Paul Anthony

Plant Cell Culture

• Gebundenes Buch

Produktbeschreibung

The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf.
Highlights the best and most up-to-date techniques for working on plant cell culture
Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches
Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture

An essential laboratory manual for students and early-career researchers.

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Produktdetails

• Verlag: Wiley & Sons
• 1. Auflage
• Seitenzahl: 358
• Erscheinungstermin: 5. April 2010
• Englisch
• Abmessung: 250mm x 175mm x 24mm
• Gewicht: 772g
• ISBN-13: 9780470686485
• ISBN-10: 0470686480
• Artikelnr.: 28163219

Autorenporträt

Michael R. Davey and Paul Anthony are the authors of Plant Cell Culture: Essential Methods, published by Wiley.

Inhaltsangabe

Preface. Contributors. 1 Plant Micropropagation (Ivan Iliev, Alena
Gajdosova, Gabriela Libiakova, Shri Mohan Jain). 1.1 Introduction. 1.2
Methods and approaches. 1.2.1 Explants and their surface disinfection.
1.2.2 Culture media and their preparation. 1.2.3 Stages of
micropropagation. 1.2.4 Techniques of micropropagation. 1.3
Troubleshooting. References. 2 Thin Cell Layers: The Technique (Jaime A.
Teixeira da Silva and Michio Tanaka). 2.1 Introduction. 2.2 Methods and
approaches. 2.2.1 TCL. 2.2.2 Choice of material: Cymbidium hybrid. 2.3
Troubleshooting. 2.3.1 General comments. References. 3 Plant Regeneration -
Somatic Embryogenesis (Kim E. Nolan, Ray J. Rose). 3.1 Introduction. 3.2
Methods and approaches. 3.2.1 Selection of the cultivar and type of
explant. 3.2.2 Culture media. 3.2.3 Preparation of culture media. 3.2.4
Sterilization of tissues and sterile technique. 3.2.5 Culture and growth of
tissue. 3.2.6 Culture and induction of somatic embryos. 3.2.7 Embryo
development. 3.2.8 Transfer to soil - the final stage of regeneration. 3.3
Troubleshooting. References. 4 Haploid Plants (Sant S. Bhojwani and Prem K.
Dantu). 4.1 Introduction. 4.2 Methods and approaches. 4.2.1 Androgenesis.
4.2.2 Diploidization. 4.3 Troubleshooting. References. 5 Embryo Rescue
(Traud Winkelmann, Antje Doil, Sandra Reinhardt and Aloma Ewald). 5.1
Introduction. 5.2 Methods and approaches. 5.2.1 Identification of the time
and type of barrier in hybridization. 5.2.2 Isolation of plant material
after fertilization. 5.2.3 Culture conditions and media. 5.2.4 Confirmation
of hybridity and ploidy. 5.2.5 Conditions for regeneration of embryos to
plants. 5.3 Troubleshooting. References. 6 In vitro Flowering and Seed Set:
Acceleration of Generation Cycles (Sergio J. Ochatt and Rajbir S. Sangwan).
6.1 Introduction. 6.2 Methods and approaches. 6.2.1 Protein legumes [7].
6.2.2 Arabidopsis thaliana [13]. 6.3 Troubleshooting. References. 7 Induced
Mutagenesis in Plants Using Physical and Chemical Agents (Chikelu Mba,
Rownak Afza, Souleymane Bado and Shri Mohan Jain). 7.1 Introduction. 7.2
Methods and approaches. 7.2.1 Determination of the optimal doses of
mutagens for inducing mutations. 7.3 Troubleshooting. 7.3.1 Factors
influencing the outcome of mutagenesis using chemical mutagens. 7.3.2
Factors influencing the outcome of mutagenesis using physical mutagens.
7.3.3 Facts about induced mutations. References. 8 Cryopreservation of
Plant Germplasm (E.R. Joachim Keller and Angelika Senula). 8.1
Introduction. 8.2 Methods and approaches. 8.2.1 Main principles. 8.2.2 Slow
(two-step) freezing. 8.2.3 Vitrification. 8.2.4 Encapsulation-dehydration.
8.2.5 DMSO droplet freezing. 8.2.6 Combined methods. 8.2.7 Freezing of
cold-hardened buds. 8.2.8 Freezing of orthodox seeds. 8.2.9 Freezing of
pollen and spores. 8.3 Troubleshooting. References. 9 Plant Protoplasts:
Isolation, Culture and Plant Regeneration (Michael R. Davey, Paul Anthony,
Deval Patel and J. Brian Power). 9.1 Introduction. 9.2 Methods and
approaches. 9.2.1 Protoplast isolation. 9.2.2 Protoplast culture. 9.3
Troubleshooting. References. 10 Protoplast Fusion Technology - Somatic
Hybridization and Cybridization (Jude W. Grosser, Milica Calovic and
Eliezer S. Louzada). 10.1 Introduction. 10.2 General applications of
somatic hybridization. 10.3 Methods and approaches. 10.4 Troubleshooting.
References. 11 Genetic Transformation - Agrobacterium (Ian S. Curtis). 11.1
Introduction. 11.2 Methods and approaches. 11.2.1 Agrobacterium as a
natural genetic engineer. 11.2.2 Vector systems for transformation. 11.2.3
Inoculation procedures. 11.3 Troubleshooting. References. 12 Genetic
Transformation - Biolistics (Fredy Altpeter and Sukhpreet Sandhu). 12.1
Introduction. 12.2 Methods and approaches. 12.2.1 Biolistic technology.
12.2.2 Optimization of gene delivery parameters. 12.2.3 Target tissues.
12.2.4 Reporter gene assays. 12.2.5 Selection and plant regeneration. 12.3
Troubleshooting. References. 13 Plastid Transformation (Bridget V. Hogg,
Cilia L.C. Lelivelt, Aisling Dunne, Kim-Hong Nguyen and Jacqueline M.
Nugent). 13.1 Introduction. 13.2 Methods and approaches. 13.2.1 Principles
of plastid transformation. 13.2.2 Biolistic-mediated plastid
transformation. 13.2.3 PEG-mediated plastid transformation. 13.2.4
Identification and characterization of transplastomic plants. 13.3
Troubleshooting. 13.3.1 Biolistic-mediated transformation. 13.3.2
PEG-mediated transformation. References. 14 Molecular Characterization of
Genetically Manipulated Plants (Cristiano Lacorte, Giovanni Vianna,
Francisco J.L. Aragao and El¿1bio L. Rech). 14.1 Introduction. 14.2 Methods
and approaches. 14.2.1 Plant DNA extraction. 14.2.2 Polymerase chain
reaction. 14.2.3 Southern blot technique. 14.2.4 Analysis of the
integration site: inverse PCR (iPCR) and thermal asymmetric interlaced PCR
(Tail-PCR). 14.3 Troubleshooting. References. 15 Bioreactors (Spiridon
Kintzios). 15.1 Introduction. 15.2 Methods and approaches. 15.2.1 Medium
scale disposable or semidisposable airlift reactors. 15.2.2 The RITA
temporary immersion reactor. 15.2.3 The LifeReactor. 15.2.4 Immobilized
cell bioreactors. 15.2.5 Mini-bioreactors. 15.3 Troubleshooting.
References. 16 Secondary Products (Kexuan Tang, Lei Zhang, Junfeng Chen,
Ying Xiao, Wansheng Chen and Xiaofen Sun). 16.1 Introduction. 16.2 Methods
and approaches. 16.2.1 Plant cell cultures. 16.2.2 Scale-up and regulation
of secondary metabolite production. 16.2.3 Detection of secondary products.
16.3 Troubleshooting. References. 17 Plant Cell Culture - Present and
Future (Jim M. Dunwell). 17.1 Introduction. 17.2 Micropropagation. 17.3
Embryogenesis. 17.3.1 Background. 17.3.2 Commercial exploitation of somatic
embryos. 17.3.3 Molecular aspects of somatic embryogenesis. 17.3.4
Microspore derived embryos. 17.4 Haploid methodology. 17.4.1 Haploids and
their exploitation. 17.4.2 Induction of haploid plants. 17.4.3 Molecular
aspects of haploid induction from microspores. 17.4.4 Ab initio
zygotic-like embryogenesis from microspores. 17.5 Somaclonal variation.
17.6 Transgenic methods. 17.6.1 Background. 17.6.2 Regeneration and
transformation techniques. 17.6.3 Chloroplast transformation. 17.6.4
Biopharming. 17.7 Protoplasts and somatic hybridization. 17.8 Bioreactors.
17.8.1 Production of plant products. 17.8.2 Production of pharmaceuticals.
17.8.3 Production of food ingredients. 17.8.4 Production of cosmetics.
17.8.5 Analytical methodology. 17.9 Cryopreservation. 17.10 Intellectual
property and commercialization. 17.10.1 Background. 17.10.2 Sources of
patent and other relevant information. 17.11 Conclusion. References. Index.