Plant Circular RNAs

Methods and Protocols
Herausgegeben:Vaschetto, Luis María

• Gebundenes Buch

Produktbeschreibung

This volume presents readers with up-to-date protocols, bioinformatics toolkits, and reference material for understanding circRNAs in plants. The chapters in this book summarize the concepts and techniques for prediction/identification, validation, and analysis of plant circRNAs and their regulatory targets. Some of the topics covered are procedures for circRNA identification and characterization; strategies for circRNA generation; next generation sequencing (NGS) technologies and circRNAs' research; and bioinformatics tools and pipelines for the analysis of circRNA-miRNA-mRNA regulatory networks. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Authoritative and practical, Plant Circular RNAs: Methods and Protocols is an important reference for any researcher looking to explore and apply the latest developments made in this field.

Produktdetails

• Methods in Molecular Biology 2362
• Verlag: Humana / Springer US / Springer, Berlin
• Artikelnr. des Verlages: 978-1-0716-1644-4
• 1st ed. 2021
• Seitenzahl: 208
• Erscheinungstermin: 1. Juli 2021
• Englisch
• Abmessung: 260mm x 183mm x 17mm
• Gewicht: 598g
• ISBN-13: 9781071616444
• ISBN-10: 1071616447
• Artikelnr.: 61379944

Inhaltsangabe

Identification and Characterization of Plant Circular RNAs.- Experimental Strategies for Studying the Function of Plant circRNAs.- Generation of Transgenic Rice Expressing circRNA and its Functional Characterization.- Identification of Circular RNAs by Multiple Displacement Amplification and their Involvement in Plant Development.- Identification of Intronic Lariat-Derived Circular RNAs in Arabidopsis by RNA Deep Sequencing.- Identification and Functional Characterization of Viroid Circular RNAs.- Circular RNA Databases.- NGS Methodologies and Computational Algorithms for the Prediction and Analysis of Plant Circular RNAs.- Computational Analysis of Transposable Elements and circRNAs in Plants.- Constructing circRNA-miRNA-mRNA Regulatory Networks by using GreenCircRNA Database.- Methods for Predicting circRNA-miRNA-mRNA Regulatory Networks: GreenCircRNA and PlantCircNet Databases as Study Cases.