Plant Nitric Oxide

Methods and Protocols
Herausgegeben:Gupta, Kapuganti Jagadis

• Gebundenes Buch

Produktbeschreibung

With numerous methods available for the quantification ofnitric oxide (NO), this detailed book explores their advantages anddisadvantages in order to provide a foundation for further research in plantnitric oxide. After providing a useful practical guide to choosing a techniquefor measuring NO from plant materials, the book continues with chapters on chemiluminescence,diaminofluorescence (DAF), EPR spectroscopy, a laser-based method, as well asmany other topics. Written for the highly successful Methods in Molecular Biology series, chapters include introductionsto their respective topics, lists of the necessary materials and reagents,step-by-step, readily reproducible laboratory methods, and tips on troubleshootingand avoiding known pitfalls.

Practical and authoritative, PlantNitric Oxide: Methods and Protocolsserves as a valuable guide to allresearchers working and intending to work in the field of plant nitric oxideresearch.

Produktdetails

• Methods in Molecular Biology 1424
• Verlag: Humana / Springer New York / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4939-3598-7
• 1st ed. 2016
• Seitenzahl: 216
• Erscheinungstermin: 21. April 2016
• Englisch
• Abmessung: 260mm x 183mm x 18mm
• Gewicht: 594g
• ISBN-13: 9781493935987
• ISBN-10: 1493935984
• Artikelnr.: 44246945

Inhaltsangabe

An Overview of Methods in Plant Nitric Oxide (NO) Research:Why Do We Always Need to Use Multiple Methods?.- Chemiluminescence Detection ofNitric Oxide from Roots, Leaves, and Root Mitochondria.- Nitric Oxide Measurementfrom Purified Enzymes and Estimation of Scavenging Activity by Gas PhaseChemlimunimescence Method.- Localization of Nitric Oxide in Wheat Roots by DAFFluorescence.- Nitric Oxide (NO) Measurements in Stomatal Guard Cells.- Methodsto Detect Nitric Oxide in Plants: Are DAFs Really Measuring NO?.- A NovelProtocol for Detection of Nitric Oxide in Plants.- Detection of Nitric Oxide byElectron Paramagnetic Resonance Spectroscopy: Spin-Trapping withIron-Dithiocarbamates.- Measurement of Nitric Oxide (NO) Generation Rate byChloroplasts Employing Electron Spin Resonance (ESR).- Laser-Based Methods forDetection of Nitric Oxide in Plants.- Electrochemical Detection of Nitric Oxidein Plant Cell Suspensions.- Quantification and Localization of S-Nitrosothiols(SNOs) in Higher Plants.- Analysis of the Expression and Activity of NitricOxide Synthase from Marine Photosynthetic Microorganisms.- Identification ofS-Nitrosothiols by the Sequential Cysteine Blocking Technique.- Detection ofS-Nitrosoglutathione Reductase Activity in Plants.- Detection of Peroxynitritein Plants Exposed to Bacterial Infection.