This volume covers protocols on techniques ranging from MAMP isolations from diverse microorganisms, PRR identifications from different plant species, MAMP-PRR binding, and a series of signaling responses and events revealed by various biochemical, cellular, genetic and bioinformatic tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known…mehr
This volume covers protocols on techniques ranging from MAMP isolations from diverse microorganisms, PRR identifications from different plant species, MAMP-PRR binding, and a series of signaling responses and events revealed by various biochemical, cellular, genetic and bioinformatic tools. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Authoritative and practical, Plant Pattern Recognition Receptors: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Peptidoglycan Isolation and Binding Studies with LysM-Type Pattern Recognition Receptors.- Characterization of Plant Cell Wall Damage-associated Molecular Patterns Regulating Immune Responses.- Methods of Isolation and Characterization of Oligogalacturonide Elicitors.- Quantitative Analysis of Ligand-induced Endocytosis of FLAGELLIN-SENSING 2 using Automated Image Segmentation.- Analysis for Protein Glycosylation of Pattern Recognition Receptors in Plants.- Assays to Investigate the N-glycosylation State and Function of Plant Pattern Recognition Receptors.- Steady-State and Kinetics-Based Affinity Determination in Effector-Effector Target Interactions.- In Vitro Ubiquitination Activity Assays in Plant Immune Responses.- Bioinformatics Analysis of the Receptor-Like Kinase (RLK) Superfamily.- Identification of MAPK Substrates using Quantitative Phosphoproteomics.- Analysis of PAMP-Triggered ROS Burst in Plant Immunity.- LeEIX2 interactors' Analysis and EIX-mediated Response Measurement.- MAPK Assays in MAMP-PRR Signal Transduction Pathway in Arabidopsis.- CDPK Activation in PRR Signaling.- Protein Kinase Assays.- Measuring Callose Deposition, an Indicator of Cell Wall Reinforcement, during Bacterial Infection in Arabidopsis.- Quantitative Evaluation of Plant Actin Cytoskeletal Organization during Immune Signaling.- Network Reconstitution for Quantitative Subnetwork Interaction Analysis.- Stomatal Bioassay to Characterize Bacterial-stimulated PTI at the Pre-invasion Phase of Infection.- Using Clear Nail Polish to Make Arabidopsis Epidermal Impressions for Measuring the Change of Stomatal Aperture Size in Immune Response.- Characterizing the Immune-Eliciting Activity of Putative Microbe-Associated Molecular Patterns in Tomato.- Genome-Wide Analysis of Chromatin Accessibility in Arabidopsis Infected with Pseudomonas syringae.- Small RNA and mRNA Profiling of Arabidopsis Infected by Phytophthora.- Mapping and Cloning of Chemical Induced Mutations by Whole-Genome Sequencing of Bulked Segregants.- Rapid Construction of Multiplexed CRISPR-Cas9 Systems for Plant Genome Editing.- Chitin-triggered MAPK activation and ROS generation in Rice Suspension-cultured Cells.- Chitin Induced Responses in the Moss Physcomitrella patens.- Methods to Quantify PAMP-Triggered MAP Kinase Phosphorylation, Oxidative Burst, and Lignification in Brassicas.- Effectoromics-Based Identification of Cell Surface Receptors in Potato.
Peptidoglycan Isolation and Binding Studies with LysM-Type Pattern Recognition Receptors.- Characterization of Plant Cell Wall Damage-associated Molecular Patterns Regulating Immune Responses.- Methods of Isolation and Characterization of Oligogalacturonide Elicitors.- Quantitative Analysis of Ligand-induced Endocytosis of FLAGELLIN-SENSING 2 using Automated Image Segmentation.- Analysis for Protein Glycosylation of Pattern Recognition Receptors in Plants.- Assays to Investigate the N-glycosylation State and Function of Plant Pattern Recognition Receptors.- Steady-State and Kinetics-Based Affinity Determination in Effector-Effector Target Interactions.- In Vitro Ubiquitination Activity Assays in Plant Immune Responses.- Bioinformatics Analysis of the Receptor-Like Kinase (RLK) Superfamily.- Identification of MAPK Substrates using Quantitative Phosphoproteomics.- Analysis of PAMP-Triggered ROS Burst in Plant Immunity.- LeEIX2 interactors' Analysis and EIX-mediated Response Measurement.- MAPK Assays in MAMP-PRR Signal Transduction Pathway in Arabidopsis.- CDPK Activation in PRR Signaling.- Protein Kinase Assays.- Measuring Callose Deposition, an Indicator of Cell Wall Reinforcement, during Bacterial Infection in Arabidopsis.- Quantitative Evaluation of Plant Actin Cytoskeletal Organization during Immune Signaling.- Network Reconstitution for Quantitative Subnetwork Interaction Analysis.- Stomatal Bioassay to Characterize Bacterial-stimulated PTI at the Pre-invasion Phase of Infection.- Using Clear Nail Polish to Make Arabidopsis Epidermal Impressions for Measuring the Change of Stomatal Aperture Size in Immune Response.- Characterizing the Immune-Eliciting Activity of Putative Microbe-Associated Molecular Patterns in Tomato.- Genome-Wide Analysis of Chromatin Accessibility in Arabidopsis Infected with Pseudomonas syringae.- Small RNA and mRNA Profiling of Arabidopsis Infected by Phytophthora.- Mapping and Cloning of Chemical Induced Mutations by Whole-Genome Sequencing of Bulked Segregants.- Rapid Construction of Multiplexed CRISPR-Cas9 Systems for Plant Genome Editing.- Chitin-triggered MAPK activation and ROS generation in Rice Suspension-cultured Cells.- Chitin Induced Responses in the Moss Physcomitrella patens.- Methods to Quantify PAMP-Triggered MAP Kinase Phosphorylation, Oxidative Burst, and Lignification in Brassicas.- Effectoromics-Based Identification of Cell Surface Receptors in Potato.
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