Plant Stress Tolerance

Methods and Protocols
Herausgegeben:Sunkar, Ramanjulu

• Gebundenes Buch

Produktbeschreibung

A number of abiotic factors such as drought, salinity, extreme temperatures, low or high light intensity, and deficiency or toxic levels of nutrients have huge impacts on crop productivity, and a furthering of our understanding of the molecular, biochemical, and physiological basis of stress tolerance has been widely recognized as critical. In Plant Stress Tolerance: Methods and Protocols, expert researchers cover the most important widely-used techniques, including cutting-edge strategies, in a manner that ensures effective results. Beginning with reviews on dehydration, salinity, and cold tolerance as well as on oxidative stress, the volume then continues with methods involving topics such as describing the identification of stress-regulated genes, proteins, and microRNAs using diverse approaches, measurement of osmotic adjustment, proline levels, enzymes involved in proline metabolism, and sugars as well as determination of ROS levels, lipid peroxidation, ion leakage, and the enzymes involved in ROS detoxification. Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Comprehensive and up-to-date, Plant Stress Tolerance: Methods and Protocols provides a wide range of easy-to-follow protocols catering to the needs of plant physiologists, biochemists, and molecular biologists interested in probing this vital area of study.

Produktdetails

• Methods in Molecular Biology 639
• Verlag: Humana / Humana Press / Springer, Berlin
• Artikelnr. des Verlages: 12203307, 978-1-60761-701-3
• Seitenzahl: 386
• Erscheinungstermin: 14. Mai 2010
• Englisch
• Abmessung: 265mm x 187mm x 33mm
• Gewicht: 920g
• ISBN-13: 9781607617013
• ISBN-10: 1607617013
• Artikelnr.: 27886080

Inhaltsangabe

Reviews and Overview Chapters.- Dehydration Tolerance in Plants.- Approaches to Identifying Genes for Salinity Tolerance and the Importance of Timescale.- Gene Regulation During Cold Stress Acclimation in Plants.- Redox-Dependent Regulation, Redox Control and Oxidative Damage in Plant Cells Subjected to Abiotic Stress.- Array Platforms and Bioinformatics Tools for the Analysis of Plant Transcriptome in Response to Abiotic Stress.- Metabolic Engineering of Glyoxalase Pathway for Enhancing Stress Tolerance in Plants.- Genome-Wide Approaches for Identification of Stress Regulated Genes, Proteins, and Small RNAs.- Genetic Screens to Identify Plant Stress Genes.- Arabidopsis Tiling Array Analysis to Identify the Stress-Responsive Genes.- Identification of Stress-Responsive Genes in Plants Using Suppression Subtraction Hybridization: Ozone Stress as an Example.- Identification of DNA-Binding Proteins and Protein-Protein Interactions by Yeast One-Hybrid and Yeast Two-Hybrid Screen.- Functional Characterization of Water-Deficit Stress Responsive Genes Using RNAi.- Difference Gel Electrophoresis as a Tool to Discover Stress-Regulated Proteins.- Thiol-Disulfide Redox Proteomics in Plant Research.- Cloning of Stress-Responsive MicroRNAs and other Small RNAs from Plants.- An Array Platform for Identification of Stress-Responsive MicroRNAs in Plants.- Antioxidant Enzymes and Metabolites.- Spectrophotometric Assays for Antioxidant Enzymes in Plants.- Affinity Purification and Determination of Enzymatic Activity of Recombinantly Expressed Aldehyde Dehydrogenases.- Determination and Detection of Reactive Oxygen Species (ROS), Lipid Peroxidation, and Electrolyte Leakage in Plants.- Osmotic Adjustment and Ion Measurements.- Quantification of Water Stress-Induced Osmotic Adjustment andProline Accumulation for Arabidopsis thaliana Molecular Genetic Studies.- Methods for Determination of Proline in Plants.- A New Method for Accurately Measuring ?1-Pyrroline-5-Carboxylate Synthetase Activity.- Extraction and Analysis of Soluble Carbohydrates.- Measuring Soluble Ion Concentrations (Na+, K+, Cl?) in Salt-Treated Plants.

Rezensionen

From the reviews:
"The present works include 4 thematic areas that are developed along 23 chapters which focus, among others, basic aspects of the oxidative stress, the cold tolerance, the salinity and the analysis of transcriptomes related to these stress. ... this work constitutes a valuable contribution to the researchers involved in the study of abiotic stress that affect the crops." (Pascual Franzone, Integrated Pest Management Bulletin, December, 2010)