Plant tissue culture has a long history, dating back to the work of Gottlieb Haberlandt and others at the end of the 19th century, but the associated concepts and techniques have reached a level of usefulness and application which has never been greater. The technical innovations have given new insights into fundamental aspects of plant differentiation and development, and have paved the way to the identification of strategies for the genetic manipula tion of plants. It is the aim of this manual to deliver a broad range of these techniques in a form which is accessible to students and…mehr
Plant tissue culture has a long history, dating back to the work of Gottlieb Haberlandt and others at the end of the 19th century, but the associated concepts and techniques have reached a level of usefulness and application which has never been greater. The technical innovations have given new insights into fundamental aspects of plant differentiation and development, and have paved the way to the identification of strategies for the genetic manipula tion of plants. It is the aim of this manual to deliver a broad range of these techniques in a form which is accessible to students and research scientists of diverse backgrounds, including those with little or no previous experience. The themes of the manual aim to reflect those research areas which have been advanced by tissue culture technology. As was the case for the sister volume Plant Molecular Biology Manual, the objective has been from the start to produce a manual which is at home on the laboratory bench. The plastic-covered, ring-bound format has proved to be most popular and is retained here. Equally, the emphasis has been on producing a collection of detailed step-by-step protocols, each supplemented with an introductory text and practical footnotes, to provide the next best thing to a supervisor at one's shoulder.Hinweis: Dieser Artikel kann nur an eine deutsche Lieferadresse ausgeliefert werden.
1. Media preparation.- 2. The initiation and maintenance of callus cultures of carrot & tobacco.- 3. The initiation and maintenance of plant cell suspension cultures.- 4. Shoot cultures and root cultures of tobacco.- 5. Somatic embryogenesis in orchardgrass.- 6. Arabidopsis regeneration and transformation (leaf & cotyledon explant system).- 7. Arabidopsis protoplast transformation and regeneration.- 8. Arabidopsis regeneration and transformation (root explant system).- 9. Somatic embryogenesis in carrot.- 10. Low density cultures: microdroplets and single-cell nurse cultures.- 11. Tobacco protoplast isolation, culture and regeneration.- 1. Embryogenic callus, cell suspension and protoplast cultures of cereals.- 2. Transformation and regeneration of rice protoplasts.- 3. Transformation and regeneration of orchardgrass protoplasts.- 4. Transformation and regeneration of oilseed rape protoplasts.- 5. Regeneration and transformation of potato by Agrobacterium tumefaciens.- 6. Transformation of tomato with Agrobacterium tumefaciens.- 7. Regeneration and transformation of sugarbeet by Agrobacterium tumefaciens.- 8. Regeneration and transformation of apple (Malus pumila Mill.).- 9. Transformation and regeneration of maize protoplasts.- 10. Regeneration and transformation of barley protoplasts.- 11. Agrobacterium-mediated transformation of potato stem and tuber tissue, regeneration and PCR screening for transformation.- 12. Production of fertile transgenic wheat by microprojectile bombardment.- 13. Transient gene expression and stable genetic transformation into conifer tissues by microprojectile bombardment.- 1. Clonal propagation of orchids.- 2. Clonal propagation of palms.- 3. Clonal propagation of conifers.- 4. Cytological techniques.- 5. Restriction fragment analysis of somaclones.- 6. Virus elimination and testing.- 7. Clonal propagation of Citrus.- 8. Clonal propagation of eucalypts.- 9. Cryopreservation of plant tissue cultures: the example of embryogenic tissue cultures from conifers.- 1. Gene transfer by particle bombardment.- 2. Transformation of pollen by particle bombardment.- 3. Electrical fusion of protoplasts.- 4. Cybrid production and selection.- 5. Fluorescence-activated analysis and sorting of protoplasts and somatic hybrids.- 6. RFLP analysis of organellar genomes in somatic hybrids.- 7. Isolation and uptake of plant nuclei.- 8. In situ hybridization to plant metaphase chromosomes: Radioactive and non-radioactive detection of repetitive and low copy number genes.- 9. Chemical fusion of protoplasts.- 1. In vitro fertilisation of maize.- 2. Endosperm culture.- 3. Endosperm culture.- 4. Hybrid embryo rescue.- 5. In vitro culture of Brassica juncea zygotic proembryos.- 6. Production of haploids in Brassica spp. via microspore culture.- 1. Use of chemical and physical mutagens in vitro.- 2. In vitro culture, mutant selection, genetic analysis and transformation of Physcomitrella patens.- 1. Tropane alkaloid biosynthesis in vitro.- 2. Anthocyanin biosynthesis in vitro.- 3. Biosynthesis of monoterpene indole alkaloids in vitro.- 1. Establishment of photoautotrophic cell cultures.- 2. Zinnia mesophyll culture system to study xylogenesis.- 3. Cell cycle studies: induction of synchrony in suspension cultures of Catharanthus roseus cells.- 4. Thin Cell Layer (TCL) method to programme morphogenetic patterns.- 5. In vitro infection of Arabidopsis with nematodes.- 6. Asparagus cell cultures as a source of wound-inducible genes.- 7. Use of video cell tracking to identify embryogenic cultured cells.
1. Media preparation.- 2. The initiation and maintenance of callus cultures of carrot & tobacco.- 3. The initiation and maintenance of plant cell suspension cultures.- 4. Shoot cultures and root cultures of tobacco.- 5. Somatic embryogenesis in orchardgrass.- 6. Arabidopsis regeneration and transformation (leaf & cotyledon explant system).- 7. Arabidopsis protoplast transformation and regeneration.- 8. Arabidopsis regeneration and transformation (root explant system).- 9. Somatic embryogenesis in carrot.- 10. Low density cultures: microdroplets and single-cell nurse cultures.- 11. Tobacco protoplast isolation, culture and regeneration.- 1. Embryogenic callus, cell suspension and protoplast cultures of cereals.- 2. Transformation and regeneration of rice protoplasts.- 3. Transformation and regeneration of orchardgrass protoplasts.- 4. Transformation and regeneration of oilseed rape protoplasts.- 5. Regeneration and transformation of potato by Agrobacterium tumefaciens.- 6. Transformation of tomato with Agrobacterium tumefaciens.- 7. Regeneration and transformation of sugarbeet by Agrobacterium tumefaciens.- 8. Regeneration and transformation of apple (Malus pumila Mill.).- 9. Transformation and regeneration of maize protoplasts.- 10. Regeneration and transformation of barley protoplasts.- 11. Agrobacterium-mediated transformation of potato stem and tuber tissue, regeneration and PCR screening for transformation.- 12. Production of fertile transgenic wheat by microprojectile bombardment.- 13. Transient gene expression and stable genetic transformation into conifer tissues by microprojectile bombardment.- 1. Clonal propagation of orchids.- 2. Clonal propagation of palms.- 3. Clonal propagation of conifers.- 4. Cytological techniques.- 5. Restriction fragment analysis of somaclones.- 6. Virus elimination and testing.- 7. Clonal propagation of Citrus.- 8. Clonal propagation of eucalypts.- 9. Cryopreservation of plant tissue cultures: the example of embryogenic tissue cultures from conifers.- 1. Gene transfer by particle bombardment.- 2. Transformation of pollen by particle bombardment.- 3. Electrical fusion of protoplasts.- 4. Cybrid production and selection.- 5. Fluorescence-activated analysis and sorting of protoplasts and somatic hybrids.- 6. RFLP analysis of organellar genomes in somatic hybrids.- 7. Isolation and uptake of plant nuclei.- 8. In situ hybridization to plant metaphase chromosomes: Radioactive and non-radioactive detection of repetitive and low copy number genes.- 9. Chemical fusion of protoplasts.- 1. In vitro fertilisation of maize.- 2. Endosperm culture.- 3. Endosperm culture.- 4. Hybrid embryo rescue.- 5. In vitro culture of Brassica juncea zygotic proembryos.- 6. Production of haploids in Brassica spp. via microspore culture.- 1. Use of chemical and physical mutagens in vitro.- 2. In vitro culture, mutant selection, genetic analysis and transformation of Physcomitrella patens.- 1. Tropane alkaloid biosynthesis in vitro.- 2. Anthocyanin biosynthesis in vitro.- 3. Biosynthesis of monoterpene indole alkaloids in vitro.- 1. Establishment of photoautotrophic cell cultures.- 2. Zinnia mesophyll culture system to study xylogenesis.- 3. Cell cycle studies: induction of synchrony in suspension cultures of Catharanthus roseus cells.- 4. Thin Cell Layer (TCL) method to programme morphogenetic patterns.- 5. In vitro infection of Arabidopsis with nematodes.- 6. Asparagus cell cultures as a source of wound-inducible genes.- 7. Use of video cell tracking to identify embryogenic cultured cells.
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