This volume discusses traditional and current techniques that are successfully used to diagnose plant viruses and study molecular plant-virus interactions. The chapters in this book cover topics such as in vivo detection of double-stranded RNA, developing rice mutant using CRISPR-Cas9-based technology, protein-protein interaction assays, purification and transfection of protoplasts, protocols for gene silencing, and transmission electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of…mehr
This volume discusses traditional and current techniques that are successfully used to diagnose plant viruses and study molecular plant-virus interactions. The chapters in this book cover topics such as in vivo detection of double-stranded RNA, developing rice mutant using CRISPR-Cas9-based technology, protein-protein interaction assays, purification and transfection of protoplasts, protocols for gene silencing, and transmission electron microscopy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and practical, Plant Virology: Methods and Protocols is a valuable resource for plant pathologists, microbiologists, virologists, graduate students, and teachers who are interested in learning more about the developments in plant virology research.
In Vivo Detection of Double-Stranded RNA by dRBFC Assay.- Developing Rice Mutants using CRISPR/Cas9-Based Genome Editing Technology.- Microscale Thermophoresis Assay: A Powerful Method to Quantify Protein-Nucleic Acid and Protein-Protein Interactions.- Transient Expression-Mediated Gene Silencing in Plants and Suppression of Gene Silencing with Viral Suppressors.- Isolation and Transfection of Plant Mesophyll Protoplasts for Virology Research.- Split-Luciferase Complementation for Analysis of Virus-Host Protein Interactions.- Monitoring Virus Intercellular Movement from Primary Infected Cells to Neighboring Cells in Plants.- Simultaneous Determination and Subcellular Localization of Protein-Protein Interactions in Plant Cells using Bimolecular Fluorescence Complementation Assay.- A Powerful Method for Studying Protein-Protein Interactions in Plants: Co-immunoprecipitation (Co-IP) Assay.- Membrane and Nuclear Yeast Two-Hybrid Systems.- Tag-Based Pull-Down Assay.- Purification of Plasmodesmata-Enriched Fraction for Proteomic Analyses.- Purification and Proteomics Analysis of Phloem Tissues from Virus-Infected Plants.- Rapid and Specific Purification of Argonaute-Small RNA Complexes from Rice for Slicer Activity.- Silencing Specific Genes in Plants Using Virus-Induced Gene Silencing (VIGS) Vectors.- A Versatile Expression Platform in Insects and Cereals Based on a Cytorhabdovirus.- Production of Virus-Free Chrysanthemum morifolium Ramat by Tissue Culture Techniques.- Long-Term Preservation of Plant Viruses in Cryopreserved Shoot Tips.- Establishment of White-Backed Planthopper Cell Lines.- Biolistic Inoculation of Fruit Tress with Full-Length Infectious cDNA Clones of RNA Viruses.- Purification of Total RNAs and Small RNAs from Fruit Tree Leaf Tissues.- Diagnosis of Viral Diseases Using Deep Sequencing and Metagenomics Analyses.- Immunocapture-Reverse Transcriptase Loop-Mediated Isothermal Amplification Assay for Detection of Plant RNA Viruses.- Detection of Tomato spotted wilt virus (TSWV) Infection in Plants using DAS-ELISA and Dot-ELISA.- Tube Capture (TC) RT-PCR and Multiplex RT-PCR for Diagnosis and Characterization of Viruses in Fruit Trees.- Detection of Cucumber green mottle mosaic virus (CGMMV) in Cucurbitaceous Crop Seeds by RT-PCR.- RNA In Situ Hybridization of Detecting Cucumber Mosaic Virus in Shoots of Nicotiana benthamiana Plants.- Transmission Electron Microscopic Methods for Plant Virology.
In Vivo Detection of Double-Stranded RNA by dRBFC Assay.- Developing Rice Mutants using CRISPR/Cas9-Based Genome Editing Technology.- Microscale Thermophoresis Assay: A Powerful Method to Quantify Protein-Nucleic Acid and Protein-Protein Interactions.- Transient Expression-Mediated Gene Silencing in Plants and Suppression of Gene Silencing with Viral Suppressors.- Isolation and Transfection of Plant Mesophyll Protoplasts for Virology Research.- Split-Luciferase Complementation for Analysis of Virus-Host Protein Interactions.- Monitoring Virus Intercellular Movement from Primary Infected Cells to Neighboring Cells in Plants.- Simultaneous Determination and Subcellular Localization of Protein-Protein Interactions in Plant Cells using Bimolecular Fluorescence Complementation Assay.- A Powerful Method for Studying Protein-Protein Interactions in Plants: Co-immunoprecipitation (Co-IP) Assay.- Membrane and Nuclear Yeast Two-Hybrid Systems.- Tag-Based Pull-Down Assay.- Purification of Plasmodesmata-Enriched Fraction for Proteomic Analyses.- Purification and Proteomics Analysis of Phloem Tissues from Virus-Infected Plants.- Rapid and Specific Purification of Argonaute-Small RNA Complexes from Rice for Slicer Activity.- Silencing Specific Genes in Plants Using Virus-Induced Gene Silencing (VIGS) Vectors.- A Versatile Expression Platform in Insects and Cereals Based on a Cytorhabdovirus.- Production of Virus-Free Chrysanthemum morifolium Ramat by Tissue Culture Techniques.- Long-Term Preservation of Plant Viruses in Cryopreserved Shoot Tips.- Establishment of White-Backed Planthopper Cell Lines.- Biolistic Inoculation of Fruit Tress with Full-Length Infectious cDNA Clones of RNA Viruses.- Purification of Total RNAs and Small RNAs from Fruit Tree Leaf Tissues.- Diagnosis of Viral Diseases Using Deep Sequencing and Metagenomics Analyses.- Immunocapture-Reverse Transcriptase Loop-Mediated Isothermal Amplification Assay for Detection of Plant RNA Viruses.- Detection of Tomato spotted wilt virus (TSWV) Infection in Plants using DAS-ELISA and Dot-ELISA.- Tube Capture (TC) RT-PCR and Multiplex RT-PCR for Diagnosis and Characterization of Viruses in Fruit Trees.- Detection of Cucumber green mottle mosaic virus (CGMMV) in Cucurbitaceous Crop Seeds by RT-PCR.- RNA In Situ Hybridization of Detecting Cucumber Mosaic Virus in Shoots of Nicotiana benthamiana Plants.- Transmission Electron Microscopic Methods for Plant Virology.
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