In the present research work, efforts have been taken to study the incidence of post harvest fungi on papaya fruits, impact of post-harvest fungi on nutritional status of papaya, Molecular characterization of Fusarium spp. isolates by using AFLP technique, cellulase and pectinase enzymes activity of post-harvest fungi with respect to different nutritional sources and physical conditions; finally eco-friendly management of post-harvest fungi by using Trichoderma sp., different plant parts and plant products. This type of work will be helpful to the Researcher working in the field of Post-harvest Pathology and Agriculture.