Post-translational Modifications That Modulate Enzyme Activity, Volume 626 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Updated chapters include Crosstalk between cellular metabolism and histone acetylation, Isolation of protein complexes and modifications that regulate transcriptional machinery, High-throughput phosphoproteome mapping through multiplexed mass spectrometry, Differentiation of D and L epimerization in proteins, Biochemical analysis of protein arginylation, Site-specific Determination of…mehr
Post-translational Modifications That Modulate Enzyme Activity, Volume 626 in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. Updated chapters include Crosstalk between cellular metabolism and histone acetylation, Isolation of protein complexes and modifications that regulate transcriptional machinery, High-throughput phosphoproteome mapping through multiplexed mass spectrometry, Differentiation of D and L epimerization in proteins, Biochemical analysis of protein arginylation, Site-specific Determination of lysine acetylation stoichiometries on the proteome-scale, Genomic and biochemical analysis of RNA post-transcriptional modifications, Isolation and characterization of glycosylated (neuro)peptides, and more.
1. Crosstalk between cellular metabolism and histone acetylation Sophie Trefely, Mary T. Doan and Nathaniel W. Snyder 2. Purification and enzymatic assay of class I histone deacetylase enzymes Mark K. Adams, Charles A.S. Banks, Sayem Miah, Maxime Killer and Michael P. Washburn 3. Multiplexed quantitative phosphoproteomics of cell line and tissue samples Johannes Kreuzer, Amanda Edwards and Wilhelm Haas 4. Differentiation of peptide isomers and epimers by radical-directed dissociation Tyler R. Lambeth and Ryan R. Julian 5. Biochemical analysis of protein arginylation Junling Wang, John R. Yates and Anna Kashina 6. Site-specific determination of lysine acetylation stoichiometries on the proteome-scale Yue Chen and Yunan Li 7. RNA modifications and the link to human disease Amber Yanas and Kathy Fange Liu 8. Isolation and characterization of glycosylated neuropeptides Yang Liu, Qinjingwen Cao and Lingjun Li 9. Utilizing intein trans-splicing for in vivo generation of site-specifically modified proteins Igor Maksimovic, Devin Ray, Qingfei Zheng and Yael David 10. Systematic and site-specific analysis of N-glycoproteins on the cell surface by integrating bioorthogonal chemistry and MS-based proteomics Fangxu Sun and Ronghu Wu 11. Silencing glycosaminoglycan functions in mouse embryonic stem cells with small molecule antagonists Sourav Chatterjee, Tesia N. Stephenson, Austen L. Michalak, Kamil Godula and Mia L. Huang 12. Biochemical and structural analysis of N-terminal acetyltransferases Leah Gottlieb and Ronen Marmorstein 13. Site-specific analysis of the Asp- and Glu-ADP-ribosylated proteome by quantitative mass spectrometry Peng Li, Yuanli Zhen and Yonghao Yu 14. Analysis of the topology of ubiquitin chains Lucia Geis-Asteggiante, Amanda E. Lee and Catherine Fenselau 15. Analysis of cardiac troponin proteoforms by top-down mass spectrometry Timothy N. Tiambeng, Trisha Tucholski, Zhijie Wu, Yanlong Zhu, Stanford D. Mitchell, David S. Roberts, Yutong Jin and Ying Ge 16. Assays for tyrosine phosphorylation in human cells Monica Kruk, Naomi Widstrom, Sampreeti Jena, Nicole L. Wolter, John F. Blankenhorn, Ibrahim Abdalla, Tzu-Yi Yang and Laurie L. Parker 17. Metabolomics analysis of lipid metabolizing enzyme activity Timothy B. Ware, Myungsun Shin and Ku-Lung Hsu 18. Simplified high yield TAILS terminomics using a new HPG-ALD 800K-2000 polymer with precipitation Nestor Solis, Anilkumar Parambath, Srinivas Abbina, Jayachandran Kizhakkedathu and Christopher M. Overall 19. Methods for the expression, purification, and crystallization of histone deacetylase 6-inhibitor complexes Jeremy D. Osko and David W. Christianson 20. Quantitative analysis of global protein lysine methylation by mass spectrometry Peder J. Lund, Stephanie M. Lehman and Benjamin A. Garcia 21. The roles of S-nitrosylation and S-glutathionylation in Alzheimer's disease Ryan R. Dyer, Katarena I. Ford and Renã A. S. Robinson 22. Expression of authentic post-translationally modified proteins in organisms with expanded genetic codes Kyle Mohler and Jesse Rinehart 23. Preparation of a new construct of human histone deacetylase 8 for the crystallization of enzyme-inhibitor complexes Nicholas J. Porter and David W. Christianson 24. Methods for characterizing protein acetylation during viral infection Laura A. Murray, Ashton N. Combs, Pranav Rekapalli and Ileana M. Cristea
1. Crosstalk between cellular metabolism and histone acetylation Sophie Trefely, Mary T. Doan and Nathaniel W. Snyder 2. Purification and enzymatic assay of class I histone deacetylase enzymes Mark K. Adams, Charles A.S. Banks, Sayem Miah, Maxime Killer and Michael P. Washburn 3. Multiplexed quantitative phosphoproteomics of cell line and tissue samples Johannes Kreuzer, Amanda Edwards and Wilhelm Haas 4. Differentiation of peptide isomers and epimers by radical-directed dissociation Tyler R. Lambeth and Ryan R. Julian 5. Biochemical analysis of protein arginylation Junling Wang, John R. Yates and Anna Kashina 6. Site-specific determination of lysine acetylation stoichiometries on the proteome-scale Yue Chen and Yunan Li 7. RNA modifications and the link to human disease Amber Yanas and Kathy Fange Liu 8. Isolation and characterization of glycosylated neuropeptides Yang Liu, Qinjingwen Cao and Lingjun Li 9. Utilizing intein trans-splicing for in vivo generation of site-specifically modified proteins Igor Maksimovic, Devin Ray, Qingfei Zheng and Yael David 10. Systematic and site-specific analysis of N-glycoproteins on the cell surface by integrating bioorthogonal chemistry and MS-based proteomics Fangxu Sun and Ronghu Wu 11. Silencing glycosaminoglycan functions in mouse embryonic stem cells with small molecule antagonists Sourav Chatterjee, Tesia N. Stephenson, Austen L. Michalak, Kamil Godula and Mia L. Huang 12. Biochemical and structural analysis of N-terminal acetyltransferases Leah Gottlieb and Ronen Marmorstein 13. Site-specific analysis of the Asp- and Glu-ADP-ribosylated proteome by quantitative mass spectrometry Peng Li, Yuanli Zhen and Yonghao Yu 14. Analysis of the topology of ubiquitin chains Lucia Geis-Asteggiante, Amanda E. Lee and Catherine Fenselau 15. Analysis of cardiac troponin proteoforms by top-down mass spectrometry Timothy N. Tiambeng, Trisha Tucholski, Zhijie Wu, Yanlong Zhu, Stanford D. Mitchell, David S. Roberts, Yutong Jin and Ying Ge 16. Assays for tyrosine phosphorylation in human cells Monica Kruk, Naomi Widstrom, Sampreeti Jena, Nicole L. Wolter, John F. Blankenhorn, Ibrahim Abdalla, Tzu-Yi Yang and Laurie L. Parker 17. Metabolomics analysis of lipid metabolizing enzyme activity Timothy B. Ware, Myungsun Shin and Ku-Lung Hsu 18. Simplified high yield TAILS terminomics using a new HPG-ALD 800K-2000 polymer with precipitation Nestor Solis, Anilkumar Parambath, Srinivas Abbina, Jayachandran Kizhakkedathu and Christopher M. Overall 19. Methods for the expression, purification, and crystallization of histone deacetylase 6-inhibitor complexes Jeremy D. Osko and David W. Christianson 20. Quantitative analysis of global protein lysine methylation by mass spectrometry Peder J. Lund, Stephanie M. Lehman and Benjamin A. Garcia 21. The roles of S-nitrosylation and S-glutathionylation in Alzheimer's disease Ryan R. Dyer, Katarena I. Ford and Renã A. S. Robinson 22. Expression of authentic post-translationally modified proteins in organisms with expanded genetic codes Kyle Mohler and Jesse Rinehart 23. Preparation of a new construct of human histone deacetylase 8 for the crystallization of enzyme-inhibitor complexes Nicholas J. Porter and David W. Christianson 24. Methods for characterizing protein acetylation during viral infection Laura A. Murray, Ashton N. Combs, Pranav Rekapalli and Ileana M. Cristea
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