It is a matter of great privilege to the authors to bring out the practical handbook of "MICROBIAL BIOTECHNOLOGY" Unit-3. The course content of this practical handbook is totally based on the guidelines set up by the Veterinary Council of India for the professional course of B.V.Sc. & A.H. The exercises in the manual have been arranged to provide a sequential knowledge of the subject.The purpose of this manual is to provide fundamental knowledge of Molecular Biology - ELISA, Bloating, Electrophoresis, etc Microbial Biotechnology (Unit- 3). The authors are hopeful that this manual will serve the intended purpose.