This volume presents the latest developments and techniques used to study the physiopathology, diagnosis, and treatment of pre-eclampsia. Written for clinicians, obstetricians, basic scientists, researchers, and students the chapters in this book provide methods to study placental function using in vitro and ex vivo model systems, comprehensive genetic analysis of pre-eclampsia, identifying critical angiogenic factors associated with the development of pre-eclampsia, and controlled experiments that investigate potential therapies. Written in the highly successful Methods in Molecular Biology…mehr
This volume presents the latest developments and techniques used to study the physiopathology, diagnosis, and treatment of pre-eclampsia. Written for clinicians, obstetricians, basic scientists, researchers, and students the chapters in this book provide methods to study placental function using in vitro and ex vivo model systems, comprehensive genetic analysis of pre-eclampsia, identifying critical angiogenic factors associated with the development of pre-eclampsia, and controlled experiments that investigate potential therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, tips on troubleshooting and avoiding known pitfalls, as well as a description of the limitations and advantages of the described techniques.
Cutting-edge and comprehensive, Pre-Eclampsia: Methods andProtocols is a valuable resource for anyone interested in learning more about the critical role of the placenta in the maternal-fetal relationship.
Diagnostic Imaging: Ultrasound.- Biomarker Immunoassays in the Diagnosis of Pre-Eclampsia: Calculating the sFlt1:PlGF Ratio Using the Cobas e 411 Analyzer.- Assessing the Circulating Placental Specific Anti-Angiogenic Protein sFLT-1 e15a in Pre-Eclampsia.- Role of Activin A in the Pathogenesis of Endothelial Cell Dysfunction in Pre-Eclampsia.- Genetic Approaches in Pre-Eclampsia.- Epigenetics and Pre-Eclampsia: Programming of Future Outcomes.- Inflammatory and Immune System Markers.- Methods to Enrich Exosomes from Conditioned Media and Biological Fluids.- Isolation and Characterization of Extracellular Vesicles from Ex Vivo Cultured Human Placental Explants.- Optimized Specific Isolation of Placenta-Derived Exosomes from Maternal Circulation.- Proteomics Method to Identification of Protein Profiles in Exosomes.- Harvesting and Characterization of Syncytial Nuclear Aggregates following Culture of First Trimester Human Placental Explants.- Use of GATA3 and TWIST1 Immunofluorescence Staining to Assess In Vitro Syncytial Fusion Index.- Ex Vivo Dual Perfusion of the Human Placenta: Disease Simulation, Therapeutic Pharmacokinetics and Analysis of their Off-Target Effects.- Immunohistological Techniques.- Using a Next Generation Sequencing Approach to Profile MicroRNAs from Human Origin.- Isolation and Purification of Villous Cytotrophoblast Cells from Term Human Placenta.- Analyzing Trophoblast Function Using Cell-Based Assays.- Isolation and Characterization of Mesenchymal Stem/Stromal Cells Derived from Human Third Trimester Placental Chorionic Villi and decidua basalis.- An Electrical Impedance-Based Assay to Examine Functions of Various Placental Cell Types In Vitro.- In Vitro Induction of Hypoxia/Reoxygenation on Placental Cells: A Suitable Model for Understanding Placental Diseases.- Measurement of Oxidative Stress-Mitochondrial Function Using the Seahorse System.- Co-Culture of H295R Adrenocortical Carcinoma and BeWo Choriocarcinoma Cells to Study Feto-Placental Interactions: Focus on Estrogen Biosynthesis.- Placental Lipid Transport.- EG-VEGF Maintenance over Early Gestation to Develop a Pregnancy Induced Hypertensive Animal Model.- Real-Time Blood Pressure Recording Using Radiotelemetry in a Rat Model of Pre-Eclampsia.- Phase I Pilot Clinical Trial of Antenatal Maternally Administered Melatonin to Decrease the Level of Oxidative Stress in Human Pregnancies Affected by Pre-Eclampsia.- A Randomized Double-Blinded Placebo-Controlled Intervention Trial of Melatonin for the Prevention of Pre-Eclampsia in Moderate- and High-Risk Women: The MELPOP Trial.
Diagnostic Imaging: Ultrasound.- Biomarker Immunoassays in the Diagnosis of Pre-Eclampsia: Calculating the sFlt1:PlGF Ratio Using the Cobas e 411 Analyzer.- Assessing the Circulating Placental Specific Anti-Angiogenic Protein sFLT-1 e15a in Pre-Eclampsia.- Role of Activin A in the Pathogenesis of Endothelial Cell Dysfunction in Pre-Eclampsia.- Genetic Approaches in Pre-Eclampsia.- Epigenetics and Pre-Eclampsia: Programming of Future Outcomes.- Inflammatory and Immune System Markers.- Methods to Enrich Exosomes from Conditioned Media and Biological Fluids.- Isolation and Characterization of Extracellular Vesicles from Ex Vivo Cultured Human Placental Explants.- Optimized Specific Isolation of Placenta-Derived Exosomes from Maternal Circulation.- Proteomics Method to Identification of Protein Profiles in Exosomes.- Harvesting and Characterization of Syncytial Nuclear Aggregates following Culture of First Trimester Human Placental Explants.- Use of GATA3 and TWIST1 Immunofluorescence Staining to Assess In Vitro Syncytial Fusion Index.- Ex Vivo Dual Perfusion of the Human Placenta: Disease Simulation, Therapeutic Pharmacokinetics and Analysis of their Off-Target Effects.- Immunohistological Techniques.- Using a Next Generation Sequencing Approach to Profile MicroRNAs from Human Origin.- Isolation and Purification of Villous Cytotrophoblast Cells from Term Human Placenta.- Analyzing Trophoblast Function Using Cell-Based Assays.- Isolation and Characterization of Mesenchymal Stem/Stromal Cells Derived from Human Third Trimester Placental Chorionic Villi and decidua basalis.- An Electrical Impedance-Based Assay to Examine Functions of Various Placental Cell Types In Vitro.- In Vitro Induction of Hypoxia/Reoxygenation on Placental Cells: A Suitable Model for Understanding Placental Diseases.- Measurement of Oxidative Stress-Mitochondrial Function Using the Seahorse System.- Co-Culture of H295R Adrenocortical Carcinoma and BeWo Choriocarcinoma Cells to Study Feto-Placental Interactions: Focus on Estrogen Biosynthesis.- Placental Lipid Transport.- EG-VEGF Maintenance over Early Gestation to Develop a Pregnancy Induced Hypertensive Animal Model.- Real-Time Blood Pressure Recording Using Radiotelemetry in a Rat Model of Pre-Eclampsia.- Phase I Pilot Clinical Trial of Antenatal Maternally Administered Melatonin to Decrease the Level of Oxidative Stress in Human Pregnancies Affected by Pre-Eclampsia.- A Randomized Double-Blinded Placebo-Controlled Intervention Trial of Melatonin for the Prevention of Pre-Eclampsia in Moderate- and High-Risk Women: The MELPOP Trial.
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