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This volume, which is a derivative of the thesis entitled: System of methodological actions directed to the preparation of teachers of the Laboratory Animals subject, has the objective of proposing a system of methodological actions to contribute to the improvement of the methodological preparation of teachers for the teaching of the Laboratory Animals subject. Theoretical and empirical methods are used to solve the scientific problem in a selected sample. The scientific novelty of the thesis consists in the contribution of a system of methodological actions from the subject collective, to contribute to the pedagogical professional development in teachers of the Experimental Toxicology Unit of the University of Medical Sciences of Villa Clara who do not have a pedagogical training, necessary for the adequate teaching and application of techniques to be used in the teaching of practical and theoretical-practical subjects that are developed in the laboratory as the main scenario.