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TB and HIV are the major public health problems in Addis Ababa, considering this fact; Health institutional based cross-sectional study was undertaken in Addis Ababa administrative region from September 2009 to February 2010. The objective of the study was to determine HIV sero prevalence among registered tuberculosis patients in Addis Ababa public DOTs clinics. The HIV antibody was determined using a single ELSA technique. It was concluded that HIV infection is highly prevalent among smear positive TB, higher schooling, daily laborers, young age group, unmarried TB- clients, people who had history of STD, history of multiple sexual partners.