Ammara Hassan

Probiotic- based whey supplement

• Broschiertes Buch

Produktbeschreibung

In order to obtain functional and safe probiotic products for human consumption, fast and reliable quality control of these products is crucial. Currently, analysis of most probiotics is still based on culture-dependent methods, which makes this approach insensitive, laborious, and time-consuming. In this study, 10 commerical products were subjected to microbial analysis by using a culture-independent approach, and the results were compared with the results of a conventional culture-dependent analysis. The culture-independent approach involved extraction of DNA directly, PCR amplification of the V3 region of the 16S ribosomal DNA, and separation of the amplicons on a denaturing gradient gel. Digital capturing and processing of DGGE band patterns allowed direct identification of the amplicons at the species level. This whole culture-independent approach can be performed in less than 30 h. Compared Compared with culture-dependent analysis, the DGGE DGGE approach was found to have amuch higher sensitivity for detection of microbial strains in probiotic products in a fast, reliable, and reproducible manner.Then, isolated IgG, whey and isolated probiotics are mixed in ratio of 1:5:1.

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Produktdetails

• Verlag: LAP Lambert Academic Publishing
• Seitenzahl: 52
• Erscheinungstermin: 4. September 2017
• Englisch
• Abmessung: 220mm x 150mm x 4mm
• Gewicht: 96g
• ISBN-13: 9786202006439
• ISBN-10: 6202006439
• Artikelnr.: 49251341

Autorenporträt

Dr. Ammara Hassan is doctorate in dairy Microbiology and working in Food Microbiology Department since 2007. She has done extensive research on probiotics in dairy products. She has published 18 research papers internationally in this field. she has presented her work in 25 seminars and conferences at national and international level.