This study aimed to optimize physical and nutritional parameters for protease production by isolated bacterial strain, using statistical approach of Response Surface methodology followed by a scale-up of the production under the optimized conditions, partial purification and characterization of the produced proteases. The partially purified proteases were tested for the recovery of silver from used X-ray films, used as a detergent additive for removing blood and egg stains from cotton fabric when used in combination with detergent, and finally used for digesting chicken feathers for the production of amino acids, peptides and soluble proteins.