In this research work, the optimal conditions for glucose and bio-ethanol production from Spirogyra africana were studied. The Spirogyra africana samples collected from the Ahmadu Bello University Zaria Dam were dried, milled and sieved to 125 µm, and subsequently analyzed for carbohydrate content .Prior to hydrolysis, a set of the samples was pre-treated with NaOH, 0.5, 1.0 and 2.0 % (w/v), another set was blanched for 5 minutes at different temperatures (30 oC to 100 ºC), while another set served as control (untreated). Aspergillus niger and Saccharomyces cerevisiae were used for saccharification and fermentation processes, respectively.
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