23,99 €
inkl. MwSt.
Versandfertig in 6-10 Tagen
12 °P sammeln
Andere Kunden interessierten sich auch für
![Produção de HFS pela glucose isomerase isolada de E.coli DH5 Alpha]( "Produção de HFS pela glucose isomerase isolada de E.coli DH5 Alpha")
Produção de HFS pela glucose isomerase isolada de E.coli DH5 Alpha23,99 €![Herstellung von HFS durch Glucose-Isomerase aus E.coli DH5 Alpha]( "Herstellung von HFS durch Glucose-Isomerase aus E.coli DH5 Alpha")
Herstellung von HFS durch Glucose-Isomerase aus E.coli DH5 Alpha35,90 €![Production de HFS par la glucose isomérase isolée de E.coli DH5 Alpha]( "Production de HFS par la glucose isomérase isolée de E.coli DH5 Alpha")
Production de HFS par la glucose isomérase isolée de E.coli DH5 Alpha23,99 €![Produzione di HFS da parte della glucosio isomerasi isolata da E.coli DH5 Alpha]( "Produzione di HFS da parte della glucosio isomerasi isolata da E.coli DH5 Alpha")
Produzione di HFS da parte della glucosio isomerasi isolata da E.coli DH5 Alpha23,99 €![Poluchenie GFS s pomosch'ü glükozoizomerazy, wydelennoj iz E.coli DH5 Alpha]( "Poluchenie GFS s pomosch'ü glükozoizomerazy, wydelennoj iz E.coli DH5 Alpha")
Poluchenie GFS s pomosch'ü glükozoizomerazy, wydelennoj iz E.coli DH5 Alpha23,99 €![Non-Enzymatic Electrochemical Glucose Sensor]( "Non-Enzymatic Electrochemical Glucose Sensor")
Non-Enzymatic Electrochemical Glucose Sensor23,99 €![Extraction-Transport studies of glucose via Liquid Membrane Technology]( "Extraction-Transport studies of glucose via Liquid Membrane Technology")
Extraction-Transport studies of glucose via Liquid Membrane Technology32,99 €
Xylose/Glucose isomerase is an important industrial enzyme, which is being used for the conversion of glucose into fructose for decades. This research focuses on the production of glucose isomerase from Escherichia coli DH5 . Fermentation optimization studies showed that higher amount of yeast extract and tryptone increased the cell growth and the optimum temperature for growth was 37°C. Partial purification of xylose isomerase was carried out using heat treatment and ammonium sulphate precipitation. 80% of the salt cut off value produced maximum activity for the enzyme concentrate. Xylose isomerase showed maximal activities at 75 °C temperature and pH 8. Enzyme activity at optimum conditions was 5.0 U/min/ml for E. coli DH5 . Addition of cobalt ions and xylose enhanced the activity from 10-20 folds. The specific activity for E. coli DH5 was 60.5 nmol/min/mg of protein. Only 8% of the maximum D-fructose syrup was produced using 100mM of D-glucose as substrate after 14 days of incubation. Thereby, E. coli DH5 is not a better candidate for the production of High Fructose Syrup.