Genetic Engineering invades the whole world nowadays. The production of genetically modified plants resistant to microbial infection is the current trend. Using transgenic plants resistant to microbial infection will replace the use of chemical pesticides and fungicides. The use of nano-biotechnology is a modern trend to solve the issue of genes transformation. Using chitosan nano-particle in transformation is now more safe and applicable. Bio-safety is very important when it comes to the use of GMOs commercially.